Increased miR-223 expression in foetal organs is a signature of acute chorioamnionitis with systemic consequences

Journal of Cellular and Molecular Medicine
JoonHo LeeBo Hyun Yoon

Abstract

Acute chorioamnionitis, frequently observed in preterm placentas, is a major risk factor for the development of infection and non-infection-related adverse perinatal outcomes. MicroRNAs play important roles in immune cell development and function as well as in the development of cancers and neurologic diseases. We sought to investigate the changes in microRNA-223 (miR-223) expression and the functional significance of the changes in miR-223 expression in foetal organs in the presence of acute chorioamnionitis. Using formalin-fixed, paraffin-embedded (FFPE) tissue samples from foetal or neonatal autopsy cases, which are the most practical option to study the changes in several organs simultaneously, miR-223 expression profiles in foetal thymus, lung and liver were compared between cases with and without acute chorioamnionitis. Total RNA was extracted from FFPE specimens and qRT-PCR was conducted. miR-223-3p expression levels in foetal thymus (2.55-fold), lung (1.93-fold) and liver (1.70-fold) were significantly higher in cases with acute chorioamnionitis than in those without. Transfection of pre-miR-223-3p in Jurkat cells and luciferase assay and ribonucleoprotein immunoprecipitation followed by qRT-PCR analysis confirmed the b...Continue Reading

References

Oct 16, 1999·American Journal of Obstetrics and Gynecology·B H YoonJ K Jun
Oct 31, 2001·Public Health Nutrition·K M Godfrey, D J Barker
Jan 18, 2003·Developmental Cell·Jun NakaeDomenico Accili
May 20, 2003·Nature·Pere PuigserverBruce M Spiegelman
Jan 8, 2004·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Raymond W RedlineUNKNOWN Society for Pediatric Pathology, Perinatal Section, Amniotic Fluid Infection Nosology Committee
Oct 29, 2004·The American Journal of Pathology·Marina BibikovaJian-Bing Fan
Nov 2, 2004·Nature Cell Biology·Peng JinStephen T Warren
Aug 6, 2005·Science·Ramesh S PillaiWitold Filipowicz
Jan 4, 2006·American Journal of Obstetrics and Gynecology·Edoardo Di NaroGiuseppe Loverro
Apr 4, 2006·Seminars in Fetal & Neonatal Medicine·Olaf Dammann, Alan Leviton
Sep 23, 2006·Developmental Biology·Baohong ZhangTodd A Anderson
Oct 28, 2006·Toxicologic Pathology·Gail Pearse
Dec 6, 2006·BMC Clinical Pathology·Andrew C HallerRichard B Everson
Aug 31, 2007·Autoimmunity·Stanford L Peng
Sep 11, 2007·American Journal of Obstetrics and Gynecology·Daniel MontenegroChong Jai Kim
Feb 19, 2008·Nature·Jonathan B JohnnidisFernando D Camargo
Feb 29, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maria RavoAlessandro Weisz
Apr 5, 2008·Biochemical and Biophysical Research Communications·Donald E KuhnTerry S Elton
Apr 9, 2008·Oncogene·S L Peng
Jun 18, 2008·Journal of Perinatology : Official Journal of the California Perinatal Association·B W Kramer
Jul 23, 2008·Nature Immunology·David BaltimoreKonstantin D Taganov
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Jan 10, 2009·Archives of Disease in Childhood. Fetal and Neonatal Edition·J V Been, L J I Zimmermann
Feb 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Miguel F SeguraEva Hernando
Jul 4, 2009·The Journal of Biological Chemistry·Irene K Guttilla, Bruce A White
Nov 4, 2009·Biochemical and Biophysical Research Communications·Larbi Kamal HasseineEmmanuel Van Obberghen
Apr 8, 2010·The Journal of Biological Chemistry·William KongJin Q Cheng
May 11, 2010·American Journal of Obstetrics and Gynecology·Steffen KunzmannBoris W Kramer
Aug 24, 2010·Clinical Chemistry·Kiyonori MiuraHideaki Masuzaki
Nov 26, 2010·Trends in Immunology·Weiming Ouyang, Ming O Li

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Methods Mentioned

BETA
PCR
immunoprecipitation
transfection
electrophoresis
Assay
acetylation

Software Mentioned

Multi Gauge
TargetScan
SPSS

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