Increased MIR31HG lncRNA expression increases gefitinib resistance in non-small cell lung cancer cell lines through the EGFR/PI3K/AKT signaling pathway

Oncology Letters
Bing WangBing Xia

Abstract

The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R) cell lines. PC9 and PC9-R cells were treated with gefitinib and, after 48 h, proliferation and apoptosis were analyzed using a Cell Counting Kit-8 (CCK-8) assay and flow cytometry. Microarray expression profiling of lncRNAs was undertaken in both PC9 and PC9-R cells, and the expression profiles were verified by reverse transcription quantitative-polymerase chain reaction. The EGFR/PI3K/AKT signaling pathway and mitochondrial apoptosis protein expression levels were assessed by western blot analysis. The PC9 cell line treated with gefitinib had a more significant effect on cell viability and apoptosis than the PC9-R cell line (P<0.05). Expression of various lncRNAs differed significantly between the two cell lines, and MIR31HG expression in particular was significantly higher in PC9-R cells. As expected, MIR31HG lncRNA knockdown sensitized PC9-R cells to gefitinib, and further experiments revealed that turning off the EGFR/PI3K/AKT signaling pa...Continue Reading

References

Feb 15, 2001·Cell Death and Differentiation·M H Harris, C B Thompson
Jul 31, 2004·Science·Raffaella SordellaJeffrey Settleman
Oct 12, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Katsuyuki HottaMitsune Tanimoto
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Andrew SlackJason M Shohet
Mar 8, 2008·Current Opinion in Genetics & Development·Jeffrey A Engelman, Jeffrey Settleman
May 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeffrey A Engelman, Pasi A Jänne
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Sep 27, 2008·Cell Cycle·Meng QiaoArthur B Pardee
Oct 5, 2010·Cell·Ulf Andersson ØromRamin Shiekhattar
Jul 20, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fu YangShu-han Sun
Nov 8, 2011·Clinics in Chest Medicine·Charles S Dela CruzRichard A Matthay
Mar 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Xiao-Hong KangMing-Juan Liao
Jun 4, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Shancheng RenYinghao Sun
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Feb 8, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mantang QiuRong Yin
Apr 12, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Li ZhangJin-Ping Zhao
May 6, 2014·Profiles of Drug Substances, Excipients, and Related Methodology·A F M Motiur RahmanMohammed Gabr Kassem
Jan 17, 2015·Oncoscience·Biagio RicciutiGiulio Metro
Apr 22, 2015·Oncotarget·Magesh MuthuArun K Rishi
Apr 23, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yunzhen GaoJingfeng Liu
Apr 25, 2015·Molecular and Cellular Biochemistry·Hui CaiYong Gao
Apr 25, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jing ZhangXiaodong Chen

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Citations

Jan 8, 2020·Current Drug Metabolism·Priya MondalSyed Musthapa Meeran
Feb 13, 2020·Journal of Cellular and Molecular Medicine·Cheng FangShiguo Zhu
Oct 3, 2018·International Journal of Oncology·Wei SunDongsheng Huang
Sep 20, 2017·Journal of Cellular Physiology·Yíng WangHai-Bo Hu
May 5, 2018·Thoracic Cancer·Leirong WangZhuang Yu
Jun 26, 2020·Clinical Genetics·He-da ZhangJin-Hai Tang
Jun 5, 2020·Cellular Oncology (Dordrecht)·Arash PoursheikhaniSadegh Babashah
Aug 24, 2018·Journal of Hematology & Oncology·Hui SunMi-Die Xu
Dec 4, 2019·International Journal of Oncology·Yuta NakanoKazuma Kishi
Aug 18, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Shuang WuLingqun Zhu
Aug 28, 2020·Cancer Gene Therapy·Manuela La MontagnaMichela Garofalo
Sep 15, 2017·Clinical Epigenetics·Abril Marcela Herrera-SolorioFederico Ávila-Moreno
Mar 14, 2020·Molecular Cancer·Kaisheng LiuZhe-Sheng Chen
May 2, 2021·Clinical and Translational Medicine·Yu ChenYouping Deng
Aug 28, 2021·International Journal of Molecular Sciences·Kuo-Wei ChangShu-Chun Lin
Jan 26, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jiabi QinRuixue Huang
Nov 28, 2021·Journal of Clinical Laboratory Analysis·Yuanfeng WeiLijuan Pang

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Methods Mentioned

BETA
transfection
Feature Extraction
immunoprecipitation
protein assay
flow cytometry
chip

Software Mentioned

Gene Spring GX
SPSS
ModFit
CellQuest [UNK] Pro

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