Increased MMP-2 activity during intervertebral disc degeneration is correlated to MMP-14 levels

The Journal of Pathology
J P H J RutgesL B Creemers

Abstract

Intervertebral disc (IVD) degeneration is associated with the increased expression of several matrix metalloproteinases (MMPs), in particular MMP-2. However, little is known about the actual activity of MMP-2 in healthy and degenerated discs, or what mechanisms are involved in its activation. A major activation pathway involves complex formation with MMP-14 and a tissue inhibitor of metalloproteinases-2 (TIMP-2). In a series of 56 human IVDs, obtained at autopsy and graded according to the Thompson score (I-V), we analysed whether MMP-2 activity was increased in different stages of IVD degeneration and to what extent activation was related to the production of MMP-14 and TIMP-2. MMP-2 activation and production were quantified by gelatin zymography. Immunohistochemical staining of MMP-14 and TIMP-2 was quantified with a video overlay-based system. A positive correlation was observed between the amount of active MMP-2 and pro-MMP-2 and degeneration grade (p < 0.001, correlation coefficient (CC) 0.557; and p < 0.001, CC 0.556, respectively). MMP-2 activity correlated positively with MMP-14 and less strongly with TIMP-2 (p = 0.001, CC 0.436; and p = 0.03, CC 0.288, respectively). Moreover, immunopositivity for MMP-14 correlated to ...Continue Reading

References

Jan 1, 1991·The Clinical Journal of Pain·G de Girolamo
Apr 1, 1997·Arthritis and Rheumatism·F H BüttnerE Bartnik
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·U Benbow, C E Brinckerhoff
May 29, 2000·The Journal of Biological Chemistry·Z WangP D Soloway
Jun 23, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·N RuangpanitE W Thompson
Oct 5, 2001·The Journal of Biological Chemistry·C J MorrisonC M Overall
Jun 27, 2002·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·H E GruberE N Hanley
Jul 12, 2002·Arthritis Research·Gillian MurphyIan Clark
Aug 24, 2002·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·C WeilerN Boos
Feb 20, 2003·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·B ShenF Taylor
May 27, 2003·Investigative Ophthalmology & Visual Science·Juha M HolopainenTimo M T Tervo
Sep 6, 2003·Cancer Cell·Kenn HolmbeckHenning Birkedal-Hansen
Nov 1, 1951·Proceedings of the Society for Experimental Biology and Medicine·M FRIEDMAN, S O BYERS
Aug 13, 2004·The Journal of Pathology·Christine Lyn Le MaitreJudith Alison Hoyland
Nov 10, 2004·Clinical Orthopaedics and Related Research·Diah S BramonoGregory H Altman
Jul 1, 2005·Arthritis Research & Therapy·Christine Lyn Le MaitreJudith Alison Hoyland
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Sally RobertsJanis Menage
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Helena Brisby
Aug 18, 2006·Spine·Michael A Adams, Peter J Roughley
Aug 29, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Cheryle A SéguinRita A Kandel
Dec 15, 2006·Journal of Cellular Physiology·Chunhong Yan, Douglas D Boyd
Jul 1, 1948·Stain Technology·H K KRISTENSEN

❮ Previous
Next ❯

Citations

Apr 13, 2010·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·J P H J RutgesW J A Dhert
Dec 19, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hsiao-yin YangLaura B Creemers
Sep 2, 2014·Advanced Drug Delivery Reviews·Gianluca FontanaAbhay Pandit
Mar 30, 2013·The Spine Journal : Official Journal of the North American Spine Society·Jillian E MayerAndrew C Hecht
Mar 5, 2013·The Spine Journal : Official Journal of the North American Spine Society·Tsung-Ting TsaiJong-Hwei S Pang
Jan 24, 2013·The Spine Journal : Official Journal of the North American Spine Society·Yong-Can HuangKeith D K Luk
Jan 19, 2016·The Spine Journal : Official Journal of the North American Spine Society·Stephanie L MillerJeffrey C Lotz
Jul 15, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wen-Jun WangJian Zhang
Dec 5, 2015·Frontiers in Aging Neuroscience·Pengbin YinPeifu Tang
Dec 2, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ming-Liang JiChen Wang
Aug 3, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ming HuangZhuo-Jing Luo
Mar 6, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Fumiyuki AraiJoji Mochida
Nov 21, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ming-liang JiChen Wang
Mar 31, 2015·Cell Proliferation·Wanli Jing, Wenxue Jiang
Aug 21, 2010·Osteoarthritis and Cartilage·J P H J RutgesL B Creemers
May 27, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wei-Lin ZhangZhuo-Jing Luo
May 31, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·B ChenF Gao
May 6, 2011·Spine·Niklas BergknutWouter J A Dhert
Dec 22, 2017·Signal Transduction and Targeted Therapy·Estelle C CollinAbhay Pandit
Jan 31, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruizhe WangWen Yuan
May 27, 2020·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Derek P ZwambagDiane E Gregory
Jun 25, 2017·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Sotiris-Spyros VamvakasDimitris Kletsas
Jan 23, 2021·European Journal of Pharmacology·Qingxin SongHongxing Shen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
J P H J RutgesW J A Dhert
European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
C WeilerN Boos
© 2021 Meta ULC. All rights reserved