Increased NaCl-induced interleukin-8 production by human bronchial epithelial cells is enhanced by the DeltaF508/W1282X mutation of the cystic fibrosis transmembrane conductance regulator gene

Cytokine
Mallory M ChanEdward D Chan

Abstract

A satisfactory model describing the airway surface fluid (ASF) in the airways of persons with cystic fibrosis (CF) remains to be established due to theoretical challenges to both the "Hydration Hypothesis" and the "Salt Hypothesis." Irrespective of these models, inhaled hypertonic saline is often used to facilitate clearance of inspissated secretions. Hypertonicity induces interleukin-8 (IL-8) expression, a potent chemokine for neutrophils. The objectives of this study were: (i) to determine the relative contribution of three potential cis-regulatory elements in the regulation of NaCl-induced IL-8 production in BEAS-2B human bronchial epithelial cells, (ii) to compare NaCl-induced IL-8 expression in IB3-1 bronchial epithelial cells, which have the DeltaF508/W1282X mutation of the CF transmembrane conductance regulator (CFTR) gene, with that in C38 cells, which are IB3-1 cells stably transfected with a truncated but functional CFTR gene, and (iii) to compare equal osmolar concentrations of NaCl and D-sorbitol in the induction of IL-8 in all three cell types. In human bronchial epithelial cells, binding sites for NFkappaB, AP-1, and NF-IL6 in the 5'-flanking region of the IL-8 promoter are necessary for optimal NaCl induction of ...Continue Reading

Citations

Apr 27, 2011·Human Molecular Genetics·Mairead Kelly-AubertAleksander Edelman
Jan 1, 2012·ISRN Inflammation·Nushjira PongnimitprasertFrançoise Braut-Boucher
Apr 13, 2011·Journal of Cell Communication and Signaling·Stefania MazzitelliClaudio Nastruzzi
Oct 12, 2010·Journal of Biomedicine & Biotechnology·Monica BorgattiClaudio Nastruzzi
May 1, 2007·Biochemical Pharmacology·Catherine VerhaegheVincent Bours
Oct 19, 2006·Biochemical and Biophysical Research Communications·Cristina CiganaPaola Melotti
Jul 31, 2013·The International Journal of Biochemistry & Cell Biology·Alix de BecdelièvrePascale Fanen
Jul 7, 2016·Mediators of Inflammation·Andrea SchnúrGuido Veit
Jun 25, 2019·Biological Reviews of the Cambridge Philosophical Society·Ángel G Valdivieso, Tomás A Santa-Coloma
Oct 12, 2007·Physiological Reviews·Maurice B BurgNatalia I Dmitrieva

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