Increased nuclear factor I/B expression in prostate cancer correlates with AR expression.

The Prostate
Jagpreet S NandaMagdalena M Grabowska

Abstract

Most prostate cancers express androgen receptor (AR), and our previous studies have focused on identifying transcription factors that modify AR function. We have shown that nuclear factor I/B (NFIB) regulates AR activity in androgen-dependent prostate cancer cells in vitro. However, the status of NFIB in prostate cancer was unknown. We immunostained a tissue microarray including normal, hyperplastic, prostatic intraepithelial neoplasia, primary prostatic adenocarcinoma, and castration-resistant prostate cancer tissue samples for NFIB, AR, and synaptophysin, a marker of neuroendocrine differentiation. We interrogated publically available data sets in cBioPortal to correlate NFIB expression and AR and neuroendocrine prostate cancer (NEPCa) activity scores. We analyzed prostate cancer cell lines for NFIB expression via Western blot analysis and used nuclear and cytoplasmic fractionation to assess where NFIB is localized. We performed co-immunoprecipitation studies to determine if NFIB and AR interact. NFIB increased in the nucleus and cytoplasm of prostate cancer samples versus matched normal controls, independent of Gleason score. Similarly, cytoplasmic AR and synaptophysin increased in primary prostate cancer. We observed strong...Continue Reading

References

Mar 1, 1992·Human Pathology·P A di Sant'Agnese
Sep 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·S E BarrieM Jarman
Jun 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·N PagéM V Govindan
Apr 30, 1999·The Prostate·P A Abrahamsson
Aug 4, 2004·Cancer Research·Ren Jie JinRobert J Matusik
Jul 8, 2005·The Prostate·Janni MirosevichRobert J Matusik
Aug 17, 2006·Cancer Research·Zongxiang ZhouAlexander Yu Nikitin
Oct 13, 2006·The EMBO Journal·Shen-Hsi YangAndrew D Sharrocks
Feb 3, 2007·Seminars in Oncology·Jeffrey S PalmgrenMark R Wakefield
Apr 3, 2007·Neoplasia : an International Journal for Oncology Research·Kirsten D MertzMark A Rubin
May 22, 2007·Biochemical and Biophysical Research Communications·Sudit S Mukhopadhyay, Jeffrey M Rosen
Dec 29, 2007·The American Journal of Surgical Pathology·Wenle Wang, Jonathan I Epstein
Apr 20, 2010·Lancet·Howard I ScherUNKNOWN Prostate Cancer Foundation/Department of Defense Prostate Cancer Clinical Trials Consortium
Apr 20, 2010·The Journal of Urology·Jonathan L WrightDaniel W Lin
Feb 22, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Tamara L LotanGeorge J Netto
Apr 19, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sean R WilliamsonLiang Cheng
Jul 19, 2011·Genes & Development·Alison L DooleyTyler Jacks
Mar 10, 2012·Clinical Genitourinary Cancer·Deliu-Victor MateiOttavio de Cobelli
May 16, 2012·Cancer Discovery·Ana AparicioSankar N Maity
Jul 14, 2012·BJU International·Heather J ChalfinMisop Han
Nov 22, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Himisha BeltranDavid M Nanus
Jan 30, 2013·Neoplasia : an International Journal for Oncology Research·Juan Miguel MosqueraMark A Rubin
Apr 4, 2013·Science Signaling·Jianjiong GaoNikolaus Schultz

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Citations

Dec 11, 2020·Molecular Cancer Therapeutics·Sarah E KohrtMagdalena M Grabowska

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