Increased organization of cytoskeleton accompanying the aging of human fibroblasts in vitro

Experimental Cell Research
E Wang, D Gundersen

Abstract

Fibroblastic cells of human origin have a limited lifespan in culture. One of the senescence-associated phenotypic changes is an increase in the abundance of cytoplasmic filaments. Human skin fibroblasts (strain 0011), derived from an 8-week-old male fetus, were passaged according to a predetermined schedule and examined at successive population doubling levels. In young rapidly growing cultures, fluorescence microscopy with NBD-Phallacidin shows a normal organization of the actin-containing fibers, microtubules and intermediate filaments, as has been described previously. At stages close to the end of the in vitro lifespan of the cell strain, large flat fibroblasts are the predominant cell type in culture. These large senescent fibroblasts contain numerous prominent actin fibers traversing the entire long axis of the cytoplasm. The fibers are often located adjacent to each other and appear to form a sheet on the ventral side of the cytoplasm. Staining of senescent cells with anti-tubulin antibody reveals an increase in the abundance of microtubules per cell and the distribution pattern is altered through the increase in the number of organization centers. Intermediate filaments are also more abundant and display tightly packed...Continue Reading

Citations

Jul 25, 2007·Biogerontology·Anund Hallén
May 1, 2007·Biogerontology·Anund Hallén
Jan 1, 1986·Mechanisms of Ageing and Development·J DominiceM Adolphe
Mar 1, 1987·Mechanisms of Ageing and Development·B M Stanulis-Praeger
Nov 1, 1990·Mechanisms of Ageing and Development·K J Pienta, D S Coffey
Jan 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·H U PüschelJ Brinckmann
Nov 18, 2000·Mechanisms of Ageing and Development·S R SandemanA W Lloyd
Jan 1, 1988·Collagen and Related Research·A AlbiniP K Müller
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C PetersonJ E Goldman
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G Ching, E Wang
Feb 1, 1987·Biological Reviews of the Cambridge Philosophical Society·S SenA Sharma
Oct 1, 1992·Experimental Dermatology·B M Stanulis-PraegerB A Gilchrest
Oct 12, 2014·Biogerontology·Florence C C TanMark P Mattson
May 8, 2009·Cellular and Molecular Life Sciences : CMLS·Eun Seong HwangHyun Tae Kang
Jun 26, 2012·Mechanisms of Ageing and Development·Karolin KlementPeter Hemmerich
Jan 10, 2009·Proteomics·Stefan ZimmermannUwe M Martens
Jul 24, 2010·Annals of the New York Academy of Sciences·You-Mie KimGyesoon Yoon
Jan 1, 1995·Connective Tissue Research·P P WebbM Benjamin
May 21, 2015·Brain Structure & Function·Ria UhlemannGolo Kronenberg
Oct 1, 1992·Annals of Medicine·K Koli, J Keski-Oja
Feb 7, 2015·Aging and Disease·Amanda Piano, Vladimir I Titorenko
Nov 26, 1986·Biochemical and Biophysical Research Communications·C Icard-LiepkalnsA Macieira-Coelho
Apr 1, 1991·International Journal of Cosmetic Science·C LafforgueJ Cotte
Sep 11, 2014·International Journal of Molecular Sciences·Edna Ribeiro-VarandasMargarida Delgado
Aug 11, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·João F PassosThomas B L Kirkwood

❮ Previous
Next ❯