Abstract
In order to study the role of Helicobacter pylori infection in gastric carcinogenesis, we have measured oxidized (carbonyls) and nitrated (nitrotyrosine-containing) proteins as markers for oxidative and nitrative stress in 216 human gastric biopsies using dot and western immunoblots and correlated the results with H. pylori, cagA status, expression of interleukin-8 and inducible nitric oxide synthase (iNOS) mRNAs, and gastric pathology. Higher levels of both oxidized and nitrated proteins were found in patients with either chronic gastritis or duodenal ulcer than in those with normal mucosa. The levels of modified proteins were significantly higher in inflamed samples infected with H. pylori, especially cagA+ strains, and in those with expression of interleukin-8 and iNOS mRNAs than in those negative for these parameters. These results indicate that infection with cagA+ H. pylori induces significant oxidative and nitrative stress in stomach mucosa, contributing to the pathogenesis of H. pylori-associated gastroduodenal diseases.
Citations
Oct 11, 2003·Journal of Gastroenterology and Hepatology·Harry Hua-Xiang Xia, Benjamin Chun-Yu Wong
May 4, 2006·International Journal of Cancer. Journal International Du Cancer·Ikuko KatoNubia Muñoz
Dec 18, 2002·Helicobacter·C P FelleyE Felley-Bosco
Feb 5, 2005·Helicobacter·Yi-Qian SunKurt Borch
Nov 23, 2005·Cell Proliferation·S J LeedhamN A Wright
Jan 16, 2007·Journal of Surgical Oncology·Matthias LechnerHerbert Tilg
Mar 18, 2008·Free Radical Research·Olha YelisyeyevaNeven Zarkovic
Nov 28, 2001·The Journal of Biological Chemistry·Arsalan S HaqqaniH Chaim Birnboim
Oct 17, 2003·World Journal of Gastroenterology : WJG·Can XuXiao-Hua Man
Sep 27, 2005·Helicobacter·Matthias LechnerHerbert Tilg
Dec 12, 2012·Bioscience, Biotechnology, and Biochemistry·Toshiki NakanoHiroshi Ohshima
Aug 21, 2015·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Yoon Ju NaMin Sun Cho
Feb 5, 2019·Journal of Food Science·Mario Estévez, Youling Xiong
Jul 28, 2004·Alimentary Pharmacology & Therapeutics·G NardoneP Malfertheiner
Jan 5, 2005·Digestive Diseases and Sciences·Hien Quoc HuynhRoss N Butler
May 3, 2011·Surgery Today·Seong-Gon KimHae-Wan Lee
Sep 21, 2004·Proteomics·Marie YeoKi-Baik Hahm
Feb 26, 2005·Infection and Immunity·Melanie IhrigJames G Fox
Jun 15, 2006·Antioxidants & Redox Signaling·Shosuke Kawanishi, Yusuke Hiraku
Oct 6, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Negin RaeiSaber Zahri
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Isabella Dalle-DonneRoberto Colombo
Jun 6, 2003·Free Radical Biology & Medicine·Masaharu MiyazawaHiromasa Ishii
Nov 10, 2006·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·G NardoneP Arcari
Nov 10, 2018·Journal of Agricultural and Food Chemistry·Thomas Van HeckeStefaan De Smet