Increased peripheral blood leukocyte subsets with regulatory phenotype in clinically stable long-term HIV-infected hemophilia patients on HAART may be beneficial and contribute to a decrease in autoimmunity.

Viral Immunology
Volker DanielG Opelz

Abstract

After initiation of highly-active antiretroviral therapy (HAART), long-term HIV-infected hemophilia patients have been shown to lose autoantibodies against CD4(+) peripheral blood leukocytes (PBL), suggesting that HAART induces autoimmunity-blocking mechanisms. We compared cytokine levels and subpopulations of lymphocytes and dendritic cells (DC) in the blood of 40 long-term HIV(+) patients with those of 13 long-term HIV(-) hemophilia patients; 23 HIV(+) patients had a detectable retroviral load. Cell subsets were determined using flow cytometry and cytokine levels were measured using ELISA. HIV(+) patients showed higher proportions of DC subpopulations with immunostimulatory phenotypes (p < 0.01), CD8(+) PBL (p < 0.001), and IL-2 (p < 0.001) and sIL-2R plasma levels (p = 0.002) than HIV(-) patients. They also exhibited increased proportions of T PBL with immunosuppressive phenotypes such as CD3(+)CD4(+)CD25(+)Foxp3(+) (p = 0.001), and CD3(+)CD8(+)CD28(-)Foxp3(+) PBL (p < 0.001), and a decreased IL-7R expression on CD3(+)CD8(+) PBL (p = 0.001) compared to HIV(-) patients. Frequencies of CD3(+)CD4(+)CD25(+) PBL producing IL-2, IL-4, IL-10, IL-12, and/or IFN-gamma, and of CD3(+)CD4(+)CD28(-) PBL secreting IL-2 and/or IL-4 were lo...Continue Reading

References

Apr 1, 1998·Nature·J Banchereau, R M Steinman
Dec 18, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·P A MacPhersonJ B Angel
Jan 11, 2003·Science·Shohei HoriShimon Sakaguchi
Jul 15, 2004·PLoS Biology·Kyra Oswald-RichterDerya Unutmaz
Jul 29, 2004·AIDS·Martyn A FrenchShelley F Stone
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jan AnderssonClaire A Chougnet
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark P EggenaHuyen Cao
Jul 13, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Pol André ApoilAntoine Blancher
Nov 30, 2005·Immunological Reviews·Sun-Kyeong Lee, Charles D Surh
Aug 15, 2006·Immunological Reviews·Qizhi Tang, Jeffrey A Bluestone
Sep 13, 2006·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Elliott M FallerPaul A MacPherson
Dec 23, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Vinodh PillaiNitin J Karandikar
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Audrey KinterAnthony S Fauci
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marielle ThewissenPiet Stinissen
Feb 13, 2008·European Journal of Immunology·Milada MahicEinar Martin Aandahl
Apr 9, 2008·European Journal of Immunology·Cristina M CostantinoDavid A Hafler

❮ Previous
Next ❯

Citations

Jan 1, 2014·BioResearch Open Access·Volker DanielGerhard Opelz

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
FACS
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.