Increased permeability of blood vessels after reversible electroporation is facilitated by alterations in endothelial cell-to-cell junctions

Journal of Controlled Release : Official Journal of the Controlled Release Society
Bostjan MarkelcMuriel Golzio

Abstract

Delivery of electric field pulses, i.e. electroporation (EP), to tissues has been shown to have a blood flow modifying effect. Indeed, the diameter of blood vessels exposed to EP is immediately reduced resulting in blood flow abrogation, followed by an increase in vascular permeability. The main cause of the increased permeability remains unknown. The aim of this study was to determine whether the in vivo effects of EP on permeability of blood vessels are linked to the permeabilization of endothelial cells' membrane (EC) and/or disruption of cell-to-cell junctions. We used a dorsal window chamber model in C57Bl/6 mice coupled with multiphoton microscopy and fluorescently labelled antibodies against PECAM-1 (CD31) to visualize endothelial cell-to-cell junctions. Clinically validated EP parameters were used and behavior of cell-to-cell junctions, in combination with leakage of 70 kDa fluorescein isothiocyanate labelled dextran (FD), was followed in time. After EP, a constriction of blood vessels was observed and correlated with the change in the shape of ECs. This was followed by an increase in permeability of blood vessels for 70 kDa FD and a decrease in the volume of labelled cell-to-cell junctions. Both parameters returned to ...Continue Reading

Citations

Oct 11, 2019·Radiology and Oncology·Maja BrloznikMaja Cemazar
Aug 9, 2020·Electromagnetic Biology and Medicine·C E Vera-TizatlA L Vera-Tizatl
Aug 18, 2020·Data in Brief·Denner Santos Dos AnjosCarlos Eduardo Fonseca-Alves
Nov 3, 2020·Frontiers in Veterinary Science·Ana NemecNataša Tozon
Jun 4, 2021·Cancer Management and Research·Yihao WangWei Xiang
May 16, 2021·Journal of Biomechanical Engineering·Zheng FangBing Zhang
Jul 23, 2021·Advanced Science·Elise P W JenkinsGeorge G Malliaras

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