Increased phosphate content in complement component C3, fibrinogen, vitronectin, and other plasma proteins in systemic lupus erythematosus: covariation with platelet activation and possible association with thrombosis

Arthritis and Rheumatism
K N EkdahlB Nilsson

Abstract

To investigate whether extracellular phosphorylation of plasma proteins takes place in vivo in patients with systemic lupus erythematosus (SLE), to determine possible correlations between phosphate levels and clinical and/or laboratory parameters, and to identify individual phosphorylated plasma proteins. Sera from SLE patients were analyzed for total amounts of protein-bound phosphate by a colorimetric technique, and for levels of beta-thromboglobulin by radioimmunoassay. In addition, the ability of these sera to activate platelets, resulting in the release of protein kinase, was tested using an assay in which platelet-rich plasma from healthy blood donors was incubated with sera or immune complexes from SLE patients. In this assay, [gamma-32P]ATP was added, and 32P-labeled C3 was quantified. Phosphate in individual proteins was detected by Western blot analysis. 32P-labeled, activated platelets were able to phosphorylate exogenously added proteins, without the addition of ATP or cations. Platelet-rich plasma from healthy blood donors became activated by sera or by polyethylene glycol-precipitated immune complexes from patients with SLE, which led to the extracellular phosphorylation of plasma proteins, exemplified in the C3 a...Continue Reading

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Citations

Mar 26, 2003·Pediatrics International : Official Journal of the Japan Pediatric Society·Ikuma FujiwaraKazuie Iinuma
Apr 13, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fabian StavenuiterMary J Heeb
Oct 27, 2016·Immunological Reviews·Daniel RicklinJohn D Lambris
Mar 22, 2018·Nature Reviews. Rheumatology·Petrus LingeEric Boilard
Feb 15, 2019·Frontiers in Immunology·Vasil V VasilevLubka T Roumenina
Jul 25, 2019·Frontiers in Immunology·Xiaobo LiuStefan W Schneider
Apr 6, 2021·British Journal of Pharmacology·Elias RawishHarald F Langer
Mar 6, 2007·Trends in Immunology·Maciej M MarkiewskiJohn D Lambris

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