Increased phosphate transport of Arabidopsis thaliana Pht1;1 by site-directed mutagenesis of tyrosine 312 may be attributed to the disruption of homomeric interactions

Plant, Cell & Environment
Elena B FontenotAaron P Smith

Abstract

Members of the Pht1 family of plant phosphate (Pi) transporters play vital roles in Pi acquisition from soil and in planta Pi translocation to maintain optimal growth and development. The study of the specificities and biochemical properties of Pht1 transporters will contribute to improving the current understanding of plant phosphorus homeostasis and use-efficiency. In this study, we show through split in vivo interaction methods and in vitro analysis of microsomal root tissues that Arabidopsis thaliana Pht1;1 and Pht1;4 form homomeric and heteromeric complexes. Transient and heterologous expression of the Pht1;1 variants, Pht1;1(Y312D), Pht1;1(Y312A) and Pht1;1(Y312F), was used to analyse the role of a putative Pi binding residue (Tyr 312) in Pht1;1 transporter oligomerization and function. The homomeric interaction among Pht1;1 proteins was disrupted by mutation of Tyr 312 to Asp, but not to Ala or Phe. In addition, the Pht1;1(Y312D) variant conferred enhanced Pi transport when expressed in yeast cells. In contrast, mutation of Tyr 312 to Ala or Phe did not affect Pht1;1 transport kinetics. Our study demonstrates that modifications to the Pht1;1 higher-order structure affects Pi transport, suggesting that oligomerization may...Continue Reading

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Citations

Nov 11, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jinlong LiuSheng Luan
Mar 1, 2018·Annual Review of Plant Biology·Li WangJinxing Lin
Sep 23, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David Massa LópezMarkus Damme
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Dec 4, 2016·Biochemical Society Transactions·Yilmaz AlguelBernadette Byrne
Apr 29, 2020·International Journal of Molecular Sciences·Alaa AllahhamAkiko Maruyama-Nakashita
Jan 9, 2021·Journal of Hazardous Materials·Amos Musyoki MawiaPeisong Hu
Nov 10, 2020·The Plant Journal : for Cell and Molecular Biology·Estela Jiménez-MoralesPlinio Guzmán
Jan 29, 2021·Plant & Cell Physiology·Yan WangChuanzao Mao
Oct 3, 2021·Molecular Pharmacology·William A MurphyKim L R Brouwer

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