Increased phosphorylation of specific rat cardiac and retinal proteins in taurine-depleted animals: isolation and identification of the phosphoproteins

Advances in Experimental Medicine and Biology
J B Lombardini

Abstract

Partial depletion of the tissue levels of taurine in the alive animal stimulates in vitro phosphorylation of both an approximately 20 kDa (94%) and an approximately 44 kDa (85%) protein present in subcellular fractions of the rat retina and heart. Tissue levels of taurine were reduced by feeding the animals 1.5% guanidinoethanesulfonic acid (GES) in their drinking water for 6 weeks. The increase in the in vitro phosphorylation of both the approximately 20 kDa and approximately 44 kDa proteins was reversed when the animals were subsequently given 1.5% taurine in their drinking water for an additional 6 weeks. Isolation, purification, tryptic digestion, and peptide sequence analysis of the retinal approximately 20 kDa phosphoprotein suggest that it is histone H2B. However, the results are ambiguous due to an impurity (< 25%) in the retinal preparation. Sequence analysis of the approximately 44 kDa phosphoprotein indicates that it is pyruvate dehydrogenase.

Citations

Aug 22, 2014·Acta Physiologica·I H LambertO H Mortensen
May 10, 2002·Nutritional Neuroscience·Julius D Militante, John B Lombardini

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