Increased plasminogen binding is associated with metastatic breast cancer cells: differential expression of plasminogen binding proteins

British Journal of Cancer
M RansonM S Baker

Abstract

Overexpression of urokinase-type plasminogen activator and its receptor correlates with metastatic capacity in breast cancer. In this study we show that the urokinase/urokinase receptor-overexpressing, metastatic human breast cancer cell line MDA-MB-231 (1) bound significantly more cell-surface plasminogen in a lysine-dependent manner and (2) was capable of generating large amounts of plasmin compared with the non-metastatic cell lines MCF-7 and T-47D. In addition, distinct plasminogen binding proteins were detected in the plasma membranes of the cell lines, suggesting heterogeneity of binding proteins. Plasminogen binding was analysed using a combination of dual-colour fluorescence flow cytometry and ligand histochemistry (for comparative and cellular localization of ligand binding), and fluorimetry (for Scatchard analysis). Apart from revealing the greater plasminogen binding capacity of MDA-MB-231 cells, flow cytometry and histochemistry also revealed that, in all three cell lines, non-viable or permeabilized cells bound significantly more plasminogen in a lysine-dependent manner than viable or non-permeabilized cells. Viable MDA-MB-231 cells bound plasminogen with moderate affinity and high capacity (Kd = 1.8 microM, recept...Continue Reading

Citations

Jun 11, 1999·Immunology and Cell Biology·M J O'Mullane, M S Baker
Nov 6, 2012·Journal of Biomedicine & Biotechnology·Lindsey A MilesRobert J Parmer
Nov 3, 2012·Journal of Biomedicine & Biotechnology·Edward F PlowRiku Das
Feb 16, 2006·Blood·Jennifer W MitchellLindsey A Miles
Jan 30, 2007·Breast Cancer Research : BCR·Gillian E StillfriedMarie Ranson
Jun 10, 2004·Arthritis and Rheumatism·Markus NapireiHans Georg Mannherz
Jul 27, 2010·Journal of Thrombosis and Haemostasis : JTH·T IwakiF J Castellino
Sep 24, 2011·Blood·Shahrzad LighvaniLindsey A Miles
Jul 29, 2011·The Journal of Biological Chemistry·Hongdong BaiRobert J Parmer
Mar 17, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anoop Singh ChauhanManoj Raje
Sep 15, 2001·British Journal of Cancer·N M Andronicos, M Ranson
Nov 20, 2014·International Journal of Molecular Sciences·Miroslava DidiasovaDariusz Zakrzewicz
Aug 19, 2007·The Biochemical Journal·David R CroucherMarie Ranson
Apr 4, 2021·International Journal of Molecular Sciences·Adam MisztaBas de Laat
Apr 4, 2021·International Journal of Molecular Sciences·Benjamin J BuckleyMichael J Kelso
Jul 17, 1998·Experimental Cell Research·M J O'Mullane, M S Baker
Jun 7, 2005·Experimental Cell Research·Jonathan Michaud-LevesqueRichard Béliveau
Aug 2, 2006·Biochemistry·Thomas HerrenEdward F Plow
May 17, 2011·Analytical Biochemistry·Malabika LahaPaul E Bock

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.