Increased production of interleukin-1beta and interleukin-1 receptor antagonist by peripheral blood mononuclear cells in undialyzed chronic renal failure

Nephron
T HiguchiK Kanmatsuse

Abstract

We investigated the cell content and production of IL-1beta and IL-1 receptor antagonist (Ra) by unstimulated and lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from 15 undialyzed patients with chronic renal failure (CRF; estimated GFR <10 ml/min), 15 patients on chronic hemodialysis (HD) and 15 healthy controls. These cytokines were measured by ELISA. The cell content of IL-1beta in freshly obtained PBMC was not detectable in any group. In contrast, that of IL-1Ra in CRF (1,807 +/- 370 pg/ml, p < 0.05) as well as in HD (1,791 +/- 151 pg/ml, p < 0.001) was significantly higher than that of the controls (907 +/- 156 pg/ml). In unstimulated cultured PBMC, spontaneous production of IL-1beta in CRF (66 +/- 13 pg/ml, p < 0.05) and in HD (81 +/- 29 pg/ml, p < 0.05) was significantly higher than that of the controls (26 +/- 3 pg/ml). In contrast, comparison of spontaneous production of IL-1Ra in the three groups was not significantly different. In LPS-stimulated PBMC, IL-1beta production in CRF (10,896 +/- 1,359 pg/ml, p < 0.01)and in HD(11,441 +/- 1,400 pg/ml, p < 0.01) was significantly higher than that of the controls (6,117 +/- 572 pg/ml). However, IL-1Ra production by LPS-stimulated PBMC in...Continue Reading

Citations

Nov 14, 2002·British Journal of Pharmacology·Carl GuévinVincent Pichette
Dec 14, 1999·Kidney International·P GoldwasserR H Barth
Sep 8, 2001·Mediators of Inflammation·M P DabrowskiS Szmigielski
Aug 3, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·K YonemuraA Hishida
Jan 25, 2005·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Terumi HiguchiKoichi Matsumoto
Mar 25, 2006·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Terumi HiguchiKoichi Matsumoto
Apr 26, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Maite R AranaSilvina S M Villanueva
Oct 6, 2006·Journal of the American Society of Nephrology : JASN·Josée MichaudVincent Pichette
Apr 5, 2014·Journal of the American Society of Nephrology : JASN·João L VianaAlice C Smith
Oct 5, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Judith NaudVincent Pichette
Dec 1, 2006·The Journal of Pharmacology and Experimental Therapeutics·Judith NaudVincent Pichette

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