Increased pulmonary RhoA expression in the nitrofen-induced congenital diaphragmatic hernia rat model

Journal of Pediatric Surgery
Hajime TakayasuHideaki Tanaka

Abstract

Persistent pulmonary hypertension remains a major cause of mortality and morbidity in cases of congenital diaphragmatic hernia (CDH). Recently, RhoA/Rho-kinase-mediated vasoconstriction has been reported to be important in the pathogenesis of pulmonary hypertension (PH). Several recent reports have described that fasudil, a potent Rho-kinase inhibitor and vasodilator, could represent a potential therapeutic option for PH. We designed this study to investigate the hypothesis that the expression level of RhoA is increased in the nitrofen-induced CDH rat model. The expression level of Wnt11, an activator of RhoA, was also evaluated. Pregnant rats were treated with or without nitrofen on gestational day 9 (D9). Fetuses were sacrificed on D17, D19 and D21 and were divided into control and CDH groups. Quantitative real-time polymerase chain reaction was performed to determine the pulmonary gene expression levels of both Wnt11 and RhoA. An immunofluorescence study was also performed to evaluate the expression and localization of RhoA. The relative mRNA expression levels of pulmonary Wnt11 and RhoA on D21 were significantly increased in the CDH group compared with the control group (p=0.016 and p=0.008, respectively). The immunofluores...Continue Reading

References

Dec 1, 1995·American Journal of Respiratory Cell and Molecular Biology·A J NorthP W Shaul
Aug 8, 1998·The Journal of Surgical Research·C P CoppolaJ R Gosche
Jul 4, 2002·Cell Biology International·Kwonseop KimElizabeth D Hay
Jan 28, 2003·The American Journal of Pathology·Jörg MeyJohn J Greer
Sep 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tetsutaro NagaokaMasahiko Oka
Oct 6, 2005·British Journal of Pharmacology·Christophe GuilluyGervaise Loirand
Feb 3, 2007·Journal of Pediatric Surgery·Nana NakazawaPrem Puri
Feb 3, 2007·Journal of Pediatric Surgery·Hajime TakayasuPrem Puri
May 13, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Noriyuki HommaMasahiko Oka
Jul 16, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nikki L JerniganThomas C Resta
Aug 12, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adeleke M BadejoPhilip J Kadowitz
Dec 17, 2009·Journal of Pediatric Surgery·Takashi Doi, Prem Puri
Jul 30, 2010·Cellular and Molecular Life Sciences : CMLS·Kenia Pedrosa NunesR Clinton Webb
Nov 17, 2010·Genes & Development·Luca GrumolatoStuart A Aaronson
Sep 29, 2012·Experimental and Molecular Pathology·Ilona SluiterRobbert J Rottier
Oct 24, 2012·Pediatric Surgery International·Massimo GarriboliAgostino Pierro
Nov 2, 2012·European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [et Al] = Zeitschrift Für Kinderchirurgie·S VijfhuizeI Reiss
Feb 13, 2014·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L SbragiaL A V Pereira
Feb 18, 2014·Journal of Paediatrics and Child Health·Lisette Leeuwen, Dominic A Fitzgerald

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Citations

Oct 4, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mathew J WongRobert P Jankov

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