Increased pyruvate orthophosphate dikinase activity results in an alternative gluconeogenic pathway in Rhizobium (Sinorhizobium) meliloti.

Microbiology
Østerås MagneTurlough M Finan

Abstract

The formation of phosphoenolpyruvate (PEP) is a major step in the gluconeogenic pathway in which tricarboxylic acid (TCA) cycle intermediates are converted to hexose sugars. In Rhizobium (now Sinorhizobium) meliloti this step is catalysed by the enzyme PEP carboxykinase (PCK) which converts oxaloacetate to PEP. R. meliloti Pck- mutants grow very poorly with TCA cycle intermediates as the sole source of carbon. Here, the isolation and mapping of suppressor mutations which allow Pck- mutants to grow on succinate and other TCA cycle intermediates is reported. Tn5 insertions which abolished the suppressor phenotype and mapped to the suppressor locus were located within the pod gene encoding pyruvate orthophosphate dikinase (PPDK). Strains carrying suppressor mutations had increased PPDK activity compared to the wild-type. The suppressor phenotype was dependent on the combined activities of malic enzyme and PPDK, which thus represent an alternative route for the formation of PEP in R. meliloti. PPDK activity was not required for symbiotic N2 fixation.

References

Aug 1, 1976·Analytical Biochemistry·R J HansenH Holzer
Jul 22, 1975·Biochemical and Biophysical Research Communications·E J Hansen, E Juni
Jan 1, 1992·Molecular & General Genetics : MGG·M NiersbachH J Hirsch
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 1, 1988·Journal of Bacteriology·T M FinanA Bottacin
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·H J Evans, H G Wood
Aug 19, 1974·Biochemical and Biophysical Research Communications·E J Hansen, E Juni
Sep 12, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R A Cooper, H L Kornberg
Nov 1, 1971·The Biochemical Journal·R E Reeves
Jul 1, 1984·Journal of Bacteriology·T M FinanE R Signer
Mar 1, 1995·Plant Molecular Biology·S UsamiJ N Burnell
Nov 1, 1993·Molecular and Biochemical Parasitology·I Bruchhaus, E Tannich
Apr 1, 1996·Journal of Bacteriology·B T Driscoll, T M Finan
May 1, 1996·Molecular and Biochemical Parasitology·L NevalainenM Müller

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Citations

Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Michael A DjordjevicUNKNOWN Sinorhizobium DNA Sequencing Consortium
Dec 19, 2006·Journal of Bacteriology·Craig StephensUrs Jenal
Jan 9, 2010·Bioscience, Biotechnology, and Biochemistry·Ikuo SatoTakaaki Fujii
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·D CapelaF Galibert
Aug 6, 2014·Canadian Journal of Microbiology·Barney A Geddes, Ivan J Oresnik
Mar 19, 2011·Journal of Experimental Botany·Chris J ChastainTony H T Nguyen
Apr 11, 2015·Nature Communications·Peter J EastmondJulian M Hibberd
Sep 25, 2016·Microbiological Research·Bhagya IyerShalini Rajkumar
Oct 8, 2014·The Journal of Biological Chemistry·Dayana Rodriguez-Contreras, Nicklas Hamilton
Oct 16, 2004·The Journal of Biological Chemistry·Marcela Varela-GómezRuy Perez-Montfort
Sep 8, 1998·FEMS Microbiology Reviews·M F Dunn

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