Increased rate of apoptosis and diminished phagocytic ability of human neutrophils infected with Afa/Dr diffusely adhering Escherichia coli strains

Infection and Immunity
Patrick BrestPaul Hofman

Abstract

The proinflammatory effect of Afa/Dr diffusely adhering Escherichia coli (Afa/Dr DAEC) strains have been recently demonstrated in vitro by showing that polymorphonuclear leukocyte (PMN) transepithelial migration is induced after bacterial colonization of apical intestinal monolayers. The effect of Afa/Dr DAEC-PMN interaction on PMN behavior has been not investigated. Because of the putative virulence mechanism of PMN apoptosis during infectious diseases and taking into account the high level of expression of the decay-accelerating factor (DAF, or CD55), the receptor of Afa/Dr DAEC on PMNs, we sought to determine whether infection of PMNs by Afa/Dr DAEC strains could promote cell apoptosis. We looked at the behavior of PMNs incubated with Afa/Dr DAEC strains once they had transmigrated across polarized monolayers of intestinal (T84) cells. Infection of PMNs by Afa/Dr DAEC strains induced PMN apoptosis characterized by morphological nuclear changes, DNA fragmentation, caspase activation, and a high level of annexin V expression. However, transmigrated and nontransmigrated PMNs incubated with Afa/Dr DAEC strains showed similar elevated global caspase activities. PMN apoptosis depended on their agglutination, induced by Afa/Dr DAEC...Continue Reading

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Citations

Dec 11, 2008·Immunologic Research·Adam D Kennedy, Frank R DeLeo
Dec 8, 2009·Nature Reviews. Microbiology·Matthew A Croxen, B Brett Finlay
Apr 16, 2005·Clinical Microbiology Reviews·Alain L Servin
Aug 24, 2006·Infection and Immunity·David J KlumppAnthony J Schaeffer
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Jun 20, 2007·FEMS Microbiology Reviews·Anton ZavialovVladimir Zav'yalov
May 5, 2012·Gut Microbes·Abigail ClementsGad Frankel
Feb 28, 2006·FEMS Microbiology Letters·Chantal Le Bouguénec, Alain L Servin
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin

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