PMID: 6980198May 1, 1982Paper

Increased rate of repair of ultraviolet-induced DNA strand breaks in mitogen stimulated lymphocytes

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
S M HamletP A Jennings

Abstract

Previous results from this laboratory have shown that phytohaemagglutinin-stimulated bovine lymphocytes exhibit a peak of ultraviolet-induced DNA repair synthesis 3 to 4 days after addition of mitogen. The level of repair synthesis was approximately tenfold higher than that in unstimulated lymphocytes. We have extended these studies to examine the rate of repair of strand breaks in U.V.-irradiated bovine lymphocytes. The extent of breakage of DNA was shown to be the same in mitogen-stimulated and unstimulated lymphocytes from two breeds of cattle, when determined by sedimentation of nucleoids on sucrose gradients. However, in mitogen-stimulated cells the time taken to repair DNA strand breaks was 6 hours compared to 12 hours in stationary phase lymphocytes after a U.V. dose of 5 J/m2. These results suggest that the increased rate of repair of strand breaks is due to the induction of enzymes involved at the post-incision stage of DNA repair. Thus the increased level of repair synthesis observed in earlier work correlates with an increased rate of repair of DNA strand breaks in phytohaemagglutinin-stimulated bovine lymphocytes.

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·U HübscherS Spadari
Apr 1, 1978·Experimental Cell Research·F H Yew, R T Johnson
Nov 1, 1977·Nucleic Acids Research·M F Lavin, C Kidson
Oct 21, 1976·Nature·P R Cook, I A Brazell
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·U BertazzoniS Spadari

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Citations

May 12, 2011·International Journal of Radiation Biology·Anna SanninoMaria Rosaria Scarfi

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