Increased renal and vascular cytosolic phospholipase A2 activity in rats with cirrhosis and ascites

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
M NiederbergerR W Schrier

Abstract

Indirect evidence suggests that the renal and vascular production of prostaglandins is increased in cirrhosis with ascites. However, the activity of the enzymes regulating the prostaglandin pathway has not been investigated in cirrhosis. The aim of the current study was to determine the activity of phospholipase A2 (PLA2), the key enzyme in the regulation of prostaglandin synthesis, in kidney and vascular tissue obtained from rats with carbon tetrachloride-induced cirrhosis and ascites (n = 9) and control rats (n = 6). PLA2 activity was assayed in vitro using [14C]arachidonyl-phosphatidylcholine (PC) and [14C]arachidonyl-phosphatidylethanolamine (PE) as substrates in the presence of Ca2+. Kidneys from cirrhotic rats had significantly higher PLA2 activity compared with control rats, with both PC and PE (35 +/- 5 and 40 +/- 6 vs. 21 +/- 2 and 26 +/- 3 pmol/mg/min, respectively; P < .05 for both). PLA2 activity was increased in the renal cortex as well as in the renal medulla. Fractionation of the kidney extracts by Mono-Q anion-exchange chromatography showed that the elution position of PLA2 activity corresponded to the cytosolic PLA2 isoform (cPLA2). Increased amounts of cPLA2 protein were found in kidney extracts immunoblotted ...Continue Reading

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Citations

Mar 21, 2001·Cellular Signalling·M F SantiagoM A Pérez-Albarsanz
Nov 25, 2003·Prostaglandins & Other Lipid Mediators·Joan Clària, Vicente Arroyo
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Jun 24, 2003·Journal of the American Society of Nephrology : JASN·Motoaki MiyazonoEthan P Carter

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