Increased replication of HIV-1 minor variants during hematopoietic stem-cell mobilization with filgrastim

The Journal of Infectious Diseases
T B CampbellDaniel R Kuritzkes

Abstract

The hypothesis that filgrastim (r-met-huG-CSF) activates replication of minor variants of human inmmunodeficiency virus type 1 (HIV-1) was tested by analysis of plasma quasi-species composition in 7 subjects in whom plasma HIV-1 RNA had increased during filgrastim treatment. Inferred phylogenetic trees of env sequences from 3 subjects during filgrastim treatment contained unique intrasubject subclusters that shared a most recent common ancestor with the baseline HIV-1 quasi species. Genotypes in the unique subclusters were not detected before filgrastim treatment, yet they composed 40%-70% of the plasma quasi species during treatment. The minority variants that appeared in 1 subject were more distantly related to plasma quasi species present 5 years before filgrastim treatment than were the majority of the pretreatment plasma quasi species. These findings provide evidence that increased HIV-1 replication during filgrastim treatment was associated with activation of HIV-1 variants that, before filgrastim treatment, were minor components of the plasma quasi species.

Citations

Sep 30, 2010·Cell Transplantation·Mariagrazia MichieliPaolo De Paoli
Jul 14, 2006·American Journal of Hematology·Maria Teresa BortolinPaolo De Paoli

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