PMID: 2106001Jan 24, 1990Paper

Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Journal of Immunological Methods
A J LesseM A Apicella

Abstract

We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.

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Citations

Jan 1, 1994·Antonie van Leeuwenhoek·J P Maskell
Oct 18, 2005·Applied Microbiology and Biotechnology·Bo XieJun-Chu Zhou
Nov 18, 2005·Applied Microbiology and Biotechnology·A BoschO Yantorno
Jan 22, 1998·Molecular & General Genetics : MGG·P AldridgeK Geider
Feb 14, 2009·Glycoconjugate Journal·Catherine J PaulEvgeny Vinogradov
Oct 25, 2012·Glycoconjugate Journal·Rosanna E B Young, Derek W Hood
May 3, 2013·Lipids·Magdalena A Karaś, Ryszard Russa
Jul 31, 2013·Journal of the American Society for Mass Spectrometry·Carlo SantambrogioRita Grandori
Apr 18, 1992·Journal of Immunological Methods·R S MunfordP D Rick
Sep 1, 1995·Research in Microbiology·E FreerE Moreno
Sep 21, 2000·Molecular Microbiology·J MartindaleC M Tang
Mar 31, 2010·The Journal of Biological Chemistry·Andries van MourikMarc M S M Wösten
Jul 18, 2002·The Journal of Experimental Medicine·Asesh BanerjeePeter A Rice

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