Increased retinol-free RBP4 contributes to insulin resistance in gestational diabetes mellitus

Archives of Gynecology and Obstetrics
Yanmin ChenDanqing Chen

Abstract

Retinol-binding protein 4 (RBP4) is a circulating retinol transporter that is strongly associated with insulin resistance. The aim of this study was to evaluate the RBP4 and retinol level in rat model of gestational diabetes mellitus and the relationship between retinol-free RBP4 (apo-RBP4), retinol-bound RBP4 (holo-RBP4) and insulin resistance. Pregnant rats were administered streptozotocin to induce diabetes. The RBP4 and retinol levels were evaluated in GDM and normal pregnant rats. After then, normal pregnant rats were divided into two groups to receive either apo-RBP4 or vehicle injection. The metabolic parameters and insulin signaling in adipose tissue, skeletal muscle and liver were determined in apo-RBP4 and control groups. Primary human adipocytes were cultured in vitro with different proportions of apo-RBP4 and holo-RBP4 for 24 h. The interaction between RBP4 and STRA6 was assessed by co-immunoprecipitation, and the expression of JAK-STAT pathway and insulin signaling were detected by Western blotting and immunofluorescence. We found increases in serum RBP4 levels and the RBP4:retinol ratio but not in the retinol levels in GDM rats. Exogenous apo-RBP4 injection attenuated insulin sensitivity in pregnant rats. In vitro...Continue Reading

References

Jun 16, 2006·The New England Journal of Medicine·Timothy E GrahamBarbara B Kahn
Apr 10, 2007·Diabetes Care·Susan Y ChuPatricia M Dietz
Jul 22, 2008·Experimental Biology and Medicine·Jordan P MillsSherry A Tanumihardjo
Feb 14, 2009·Diabetes, Obesity & Metabolism·C ErikstrupB K Pedersen
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniel C BerryNoa Noy
May 26, 2011·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Mariusz KuzmickiJacek Szamatowicz
Jul 12, 2011·Metabolism: Clinical and Experimental·Weerapan KhovidhunkitThipaporn Tharavanij
Feb 7, 2012·The Journal of Biological Chemistry·Chao-Hung ChenShyi-Jang Shin
Jul 11, 2012·Experimental Diabetes Research·Yuri Karen SinzatoDébora Cristina Damasceno
Jul 21, 2012·The Journal of Membrane Biology·Riki KawaguchiHui Sun
Jul 24, 2013·Journal of Diabetes Research·D C DamascenoM V C Rudge
Sep 24, 2013·Molecular and Cellular Endocrinology·Martha Lappas
Jan 15, 2014·Diabetic Medicine : a Journal of the British Diabetic Association·G E TutinoR C W Ma
Apr 16, 2014·The Lancet. Diabetes & Endocrinology·Mathias FasshauerMichael Stumvoll
May 8, 2014·Molecular and Cellular Biology·Ming ZhongHui Sun
May 8, 2014·Molecular and Cellular Biology·Noa Noy
Nov 12, 2014·Archives of Gynecology and Obstetrics·Maria João Fonseca, Ana Cristina Santos
Jul 24, 2015·Paediatric and Perinatal Epidemiology·Jia PuLatha P Palaniappan
Jan 6, 2016·The Proceedings of the Nutrition Society·Noa Noy
Nov 1, 2016·Diabetes·Spencer J ThompsonTimothy E Graham

❮ Previous
Next ❯

Citations

Sep 14, 2019·Canadian Journal of Physiology and Pharmacology·Yun LiuFutian Tang
Mar 9, 2021·Frontiers in Endocrinology·Yunting ZhouJianhua Ma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.