Increased S100A15 expression and decreased DNA methylation of its gene promoter are involved in high metastasis potential and poor outcome of lung adenocarcinoma

Oncotarget
Yung-Che ChenJen-Chieh Chang

Abstract

This study aims to determine the functional role of S100A15 and its promoter DNA methylation patterns in lung cancer progression. We analyzed 178 formalin-fixed paraffin embedded specimens from lung cancer patients, including 24 early stage and 91 advanced stage adenocarcinoma. S100A15 protein expression was evaluated by immunohistochemistry stain, and its DNA methylation levels were measured by pyrosequencing. S100A15 nuclear staining was increased in lung adenocarcinoma patients with distant metastasis versus those without distant metastasis. There was reduced one/three-year overall survival in adenocarcinoma patients receiving first line target therapy and harboring high nuclear expressions of S100A15. Both DNA methylation levels over -423 and -248 CpG sites of the S100A15 gene promoter were decreased in adenocarcinoma patients with distant metastasis, and the former was associated with lower one-year overall survival. The highly invasive CL1-5 cell lines display decreased DNA methylation over -412/-248/-56 CpG sites of the S100A15 gene promoter and increased S100A15 gene/protein expressions as compared with the less invasive CL1-0 cell lines. Knockdown of S100A15 in CL1-5 cell line inhibited cell proliferation, migration, a...Continue Reading

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Citations

Sep 8, 2018·Journal of Cellular Physiology·Chongyu SuZhidong Liu
Jul 30, 2020·Cancers·Chantal AllgöwerJohannes Lemke
Jul 6, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Ziqi MengXiaomeng Zhang
Jul 10, 2021·Pulmonary Medicine·Zeeshan SattarPatrick Geraghty

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Datasets Mentioned

BETA
GSE86344

Methods Mentioned

BETA
transfection
nuclear translocation
nuclear
biopsy
PCR
flow cytometry
electrophoresis

Software Mentioned

DESeq
Pathway Studio
Ariadne
SPSS
MRI Wound Healing Tool
Adobe PHOTOSHOP CS6

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