Increased salinity triggers significant changes in the functional proteins of ANAMMOX bacteria within a biofilm community

Chemosphere
Yabing MengFangang Meng

Abstract

Anaerobic ammonium oxidation (ANAMMOX) processes can potentially be influenced by salinity related to variable salinity in water environment. Here, we used 16S rRNA sequencing analysis combining with iTRAQ-based quantitative proteomic approach to reveal the response of microbial community and functional proteins to salinity, which was increased from 0 to 20 g L-1 with a step of 5 g L-1 (designed as S5, S10, S15 and S20) compared to control reactor (without salinity stress desined as S0). The 16S rRNA sequencing analysis showed that a high salinity (20 g L-1, S20) decreased the abundance of genus Candidatus Jettenia but increased that of Candidatus Kuenenia. A total of 1609 differentially expressed proteins were acquired in the three comparison groups (S5:S0, S20:S0 and S20:S5). Of these, 39 proteins co-occurred in the three salt-exposed samples. Hydrazine dehydrogenase (HDH; Q1PW30) and nitrate reductase (Q1PZD8) were up-regulated more than 3-folds in the exposure of 20 g-NaCl/L. The functional enrichment analysis further showed that some proteins responsible for ion binding, catalysis and oxidation-reduction reaction were up-regulated, which explained the physiological resilience of ANAMMOX bacteria under salinity stress. Addi...Continue Reading

Citations

Jan 29, 2019·Journal of Industrial Microbiology & Biotechnology·Han WangJi-Xiang Yang
Sep 24, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Lushen ZuoJianhua Guo
May 18, 2020·The Science of the Total Environment·Jiaping LiJiao Lv

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