PMID: 9166748Jun 1, 1997Paper

Increased secretion of the amino-terminal fragment of amyloid precursor protein in brains of rats with a constitutive up-regulation of protein kinase C

Journal of Neurochemistry
A CaputiMonica Di Luca

Abstract

Protein kinase C (PKC) activation stimulates release of secreted amyloid precursor protein (APPs) in several cell lines. To ascertain the role of PKC in regulating APP metabolism in vivo, we used an animal model (methylazoxymethanol-treated rats; MAM rats) in which PKC is permanently hyperactivated in selected brain areas, i.e., cortex and hippocampus. A significant decrease in membrane-bound APP concentration was found in synaptosomes derived from cortex and hippocampus of MAM rats, where PKC is up-regulated, with a concomitant increase in APPs production in soluble fractions of the same brain areas. In contrast, in a brain area not affected by MAM treatment (i.e., cerebellum), APP secretion is similar in control and MAM rats, indicating that altered metabolism of APP is restricted to only those areas in which the PKC system is up-regulated. In addition, phorbol esters or H-7 modulate APPs release in hippocampal slices from both control and MAM rats, further supporting an in vivo role for this enzyme in regulating metabolism of mature APP.

Citations

Feb 26, 1999·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·H L HedinC J Fowler
May 6, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S Rossner
Feb 4, 1999·Journal of Neurochemistry·J Mills, P B Reiner
May 5, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S RossnerV Bigl
Mar 2, 2006·Drugs & Aging·Martina ZimmermannMonica Di Luca
May 17, 2011·Reviews in the Neurosciences·Karim BordjiAlain Buisson
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·K T ShawN H Greig
Mar 3, 2004·Brain Research. Molecular Brain Research·Wiep ScheperFrank Baas
Dec 2, 2004·Journal of Neuroscience Research·Moussa B H YoudimTamar Amit
Apr 27, 2005·Brain Research. Brain Research Reviews·Silvia MandelMoussa B H Youdim

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