PMID: 9443983Mar 14, 1998Paper

Increased sensitivity and reproducibility of TRAP assay by avoiding direct primers interaction

Nucleic Acids Research
M L FalchettiE D'Ambrosio

Abstract

Telomeric Repeat Amplification Protocol (TRAP) is a sensitive procedure to measure telomerase activity in small samples of cell or tissue extracts. Due to the strict correlation between high levels of telomerase activity and neoplastic transformation, TRAP assay could provide an important diagnostic marker of malignancy. Although the original TRAP assay is very sensitive and some improvements have been described, occasional artifacts still persist in the modified procedures. Here we describe how changes in the sequence of the primer used for the amplification step enhance the reproducibility and sensitivity in the TRAP assay.

Citations

Nov 28, 2000·International Journal of Cancer. Journal International Du Cancer·M L FalchettiL M Larocca
Apr 18, 2007·Analytical and Bioanalytical Chemistry·Deming KongHanxi Shen
Jan 30, 2004·Journal of Cancer Research and Clinical Oncology·Carlo VicentiniMauro Bologna
Feb 28, 2004·Biochimie·P A FradianiP Donini
Jul 23, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Tzu-Chin WuJiunn-Liang Ko
Aug 18, 1999·Journal of Clinical Pathology·M L FalchettiE D'Ambrosio
Jul 1, 2006·Neurological Research·Maria Laura FalchettiRoberto Pallini
Feb 14, 2002·Nucleic Acids Research·Jean-Louis MergnyEric Gilson
May 12, 2000·European Journal of Biochemistry·T Sasaki, H Fujiwara
Dec 7, 2005·International Journal of Cancer. Journal International Du Cancer·Roberto PalliniMaria Laura Falchetti
May 9, 2006·Experimental Gerontology·Christine FehrerGünter Lepperdinger
Jan 4, 2006·Experimental Cell Research·Regina VoglauerJohannes Grillari
Apr 4, 2003·Analytical Biochemistry·Sabita N SaldanhaTrygve O Tollefsbol
Jan 28, 2004·Cancer Research·Jan GrimmRalph Weissleder

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