Increased sensitivity of apolipoprotein E knockout mice to copper-induced oxidative injury to the liver

Biochemical and Biophysical Research Communications
Yuan ChenLi Guo

Abstract

Apolipoprotein E (ApoE) genotypes are related to clinical presentations in patients with Wilson's disease, indicating that ApoE may play an important role in the disease. However, our understanding of the role of ApoE in Wilson's disease is limited. High copper concentration in Wilson's disease induces excessive generation of free oxygen radicals. Meanwhile, ApoE proteins possess antioxidant effects. We therefore determined whether copper-induced oxidative damage differ in the liver of wild-type and ApoE knockout (ApoE(-/-)) mice. Both wild-type and ApoE(-/-) mice were intragastrically administered with 0.2 mL of copper sulfate pentahydrate (200 mg/kg; a total dose of 4 mg/d) or the same volume of saline daily for 12 weeks, respectively. Copper and oxidative stress markers in the liver tissue and in the serum were assessed. Our results showed that, compared with the wild-type mice administered with copper, TBARS as a marker of lipid peroxidation, the expression of oxygenase-1 (HO-1), NAD(P)H dehydrogenase, and quinone 1 (NQO1) significantly increased in the ApoE(-/-) mice administered with copper, meanwhile superoxide dismutase (SOD) activity significantly decreased. Thus, it is concluded that ApoE may protect the liver from co...Continue Reading

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Citations

May 11, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yifu ZhuangKerong Dai
Feb 15, 2019·Life Science Alliance·Eloise HudryBradley T Hyman
Aug 29, 2018·Journal of Bacteriology·Susan P Gardner, Jonathan W Olson

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