Increased sensitivity of EBNA2-transformed rat fibroblasts to ionizing radiation

International Journal of Cancer. Journal International Du Cancer
M ShimakageA Hakura

Abstract

To clarify whether expression of Epstein-Barr virus(EBV) nuclear antigen 2 (EBNA2) correlates with sensitivity to ionizing radiation, we tested EBNA2-transformed rat fibroblast clones for their radiosensitivity to X-rays. These transformed clones reproducibly generated tumors in mice. X-irradiation suppressed the growth of the tumors, and irradiated mice survived longer than non-irradiated ones. In contrast, tumors formed by activated H-ras or E6-E7 genes of human papillomavirus type 16 (HPV16) were strongly resistant to the same dose of X-irradiation. In in vitro culture, these EBNA2-expressing clones also showed higher radiosensitivity than cell lines transformed by activated H-ras and E6E7 genes. The averaged D(o) of EBNA2-expressing clones was 2.3 times lower than that of non-expressing and control clones. These results suggest that expression of EBNA2 is responsible for the radiosensitivity.

References

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Citations

May 30, 2009·International Journal of Radiation Biology·Ping-Kun ZhouJing An
Apr 4, 2003·Journal of the American College of Nutrition·Kedar N PrasadWilliam C Cole
Nov 17, 2005·Cancer Research·Haritha K D L ReddyE Premkumar Reddy

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