PMID: 11900853Mar 20, 2002Paper

Increased sensitivity to NMDA is involved in alcohol-withdrawal induced cytotoxicity observed in primary cultures of cortical neurones chronically pre-treated with ethanol

Neurochemistry International
József Nagy, L László

Abstract

Severe cellular damage and neuronal cell loss were previously observed in cultures of primary cortical neurones after chronic ethanol pre-treatment followed by ethanol-withdrawal. In this study, we investigated the circumstances and the possible cellular changes leading to alcohol-withdrawal induced neuronal cell death. When cultures were pre-treated with ethanol (25-200mM) once for 24 or 72h, the amount of the subsequent 24h alcohol-withdrawal induced cell death-estimated by measuring the release of lactate dehydrogenase (LDH)-was elevated only in cultures pre-treated with 200mM ethanol for 72h. On the contrary, as little as 50mM ethanol produced significant (P<0.01) increase in the withdrawal induced LDH-release in cultures pre-treated repeatedly with ethanol once daily for three consecutive days. When ethanol was re-added to the cultures during the withdrawal period, the LDH-release was dose-dependently reduced to the level of control. In ethanol pre-treated cultures N-methyl-D-aspartate (NMDA) (0.01-1mM) induced excitotoxicity as well as NMDA evoked elevation of cytosolic calcium ion concentration was increased. In contrast, the depolarising agent veratridine (0.01-1mM) produced similar extent of neuronal injury and elevati...Continue Reading

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Citations

Jan 14, 2003·European Journal of Pharmacology·Abdelkader Dahchour, Philippe De Witte
Feb 7, 2012·Neuropharmacology·Rashna BalsaraFrancis J Castellino
Sep 29, 2011·Micron : the International Research and Review Journal for Microscopy·Hanmei QiuPeng Liu
Jan 28, 2004·Alcoholism, Clinical and Experimental Research·Tara A Lindsley, Shannon Clarke
May 15, 2003·British Journal of Pharmacology·Hui-Fang Li, Joan J Kendig
May 20, 2015·Theoretical Biology & Medical Modelling·Carlos A Gutierrez, Mary M Staehle
Aug 5, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Mei QiangJi Li
Jun 10, 2016·The Journal of Pharmacology and Experimental Therapeutics·Marianna E JungHriday K Das

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