Increased sensitivity using real-time dPCR for detection of SARS-CoV-2.

BioTechniques
Kyra DuongYan Wang

Abstract

A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end point dPCR compared with real-time qPCR, and the limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals. Real-time dPCR showed increased linear dynamic range compared with end point dPCR based on quantitation from amplification curves. Real-time dPCR can improve the performance of TaqMan assays beyond real-time qPCR and end point dPCR with better sensitivity and specificity, absolute quantification and a wider linear range of detection.

References

Jun 3, 2004·The Lancet Infectious Diseases·Samuel Yang, Richard E Rothman
Aug 3, 2005·BioTechniques·Marisa L Wong, Juan F Medrano
Dec 12, 2012·Journal of Clinical Microbiology·R T HaydenA M Caliendo
Sep 3, 2013·Nature Methods·Christopher M HindsonMuneesh Tewari
Aug 20, 2014·Biomolecular Detection and Quantification·Coren A MilburyBrian Hutchison
Dec 19, 2016·Biomolecular Detection and Quantification·Gerwyn M JonesAlexandra S Whale

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Methods Mentioned

BETA
PCR
chip
nucleic acid amplification
chips

Software Mentioned

QuantStudio 3D Analysis Suite Cloud
Gnomegen
Fluidigm
QuantStudio 3D
Graph Pad Prism
QuantStudio

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