Increased serum levels of microvesicles in nonvalvular atrial fibrillation determinated by ELISA using a specific monoclonal antibody AD-1

Clinica Chimica Acta; International Journal of Clinical Chemistry
Hua WangWei Zhang

Abstract

Microvesicles are involved in different pathological processes such as inflammation, coagulation and tumor progression. We intended to establish an immunoaffinity capture method for detecting microvesicles and bioactive effectors carried on them using a specific homemade monoclonal antibody AD-1. By this method we investigated the association of inflammation with platelet activation in patients with nonvalvular atrial fibrillation (NVAF). A case-control study of 90 Chinese subjects selected in 3 groups: control, paroxysmal AF, and persistent AF. After capturing the microvesicles of serum using a specific monoclonal antibody AD-1, the amounts of LAP, IL-1β and P-selectin loaded on these microvesicles were quantified by either enzyme activity assay (LAP) or ELISA respectively. Compared with normal controls, the patients with persistent AF showed significantly increased serum levels of microvesicles (P<0.001), microvesicle-bound IL-1 β (P=0.019) and microvesicle-bound P-selectin (P=0.001). The latter two were significantly correlated with each other (r(2)=0.371, r=0.616, P<0.001). The microvesicle-bound IL-1β (β=0.570, P<0.001) and body weight (β=0.427, P=0.002) were as independent predictors of platelet activation. The method was...Continue Reading

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Citations

Jan 28, 2015·Nature Reviews. Cardiology·Yu-Feng HuShih-Ann Chen
Sep 3, 2016·Biomarkers in Medicine·Åsa ThulinAgneta Siegbahn
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Sep 1, 2021·Advanced Science·Li MinShutao Wang

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