Increased spinal dynorphin levels and phospho-extracellular signal-regulated kinases 1 and 2 and c-Fos immunoreactivity after surgery under remifentanil anesthesia in mice

Molecular Pharmacology
Ana CampilloMargarita M Puig

Abstract

In humans, remifentanil anesthesia enhances nociceptive sensitization in the postoperative period. We hypothesized that activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and the expression of c-Fos, prodynorphin (mRNA), and dynorphin in the spinal cord could participate in the molecular mechanisms underlying postoperative opioid-induced sensitization. In a mouse model of incisional pain, we evaluated thermal (Hargreaves test) and mechanical (von Frey) hyperalgesia during the first 21 postoperative days. Moreover, prodynorphin (mRNA, real-time polymerase chain reaction), dynorphin (enzymatic immunoassay), c-Fos expression, and ERK1/2 phosphorylation (both by immunohistochemistry) in the lumbar spinal cord were assessed. Surgery performed under remifentanil anesthesia induced a maximal decrease in nociceptive thresholds between 4 h and 2 days postoperatively (p < 0.001) that lasted 10 to 14 days compared with noninjured animals. In the same experimental conditions, a significant increase in prodynorphin mRNA expression (at 2 and 4 days) followed by a sustained increase of dynorphin (days 2 to 10) in the spinal cord was observed. We also identified an early expression of c-Fos immunoreactivity in the superficia...Continue Reading

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Citations

Aug 23, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F M TeixeiraM A Medeiros
Jun 4, 2013·The Journal of Pain : Official Journal of the American Pain Society·Angela MacalusoSaul Collini
Oct 11, 2011·Peptides·Richard J Bodnar
Feb 9, 2011·European Journal of Pharmacology·Ana CampilloMargarita María Puig
Feb 12, 2013·European Journal of Pharmacology·Asunción RomeroMargarita M Puig
Jan 16, 2015·Nature Reviews. Neuroscience·Stéphane Mélik ParsadaniantzAnnabelle Réaux-Le Goazigo
Jun 14, 2016·The Journal of Pharmacy and Pharmacology·Elizabeth Romero-AlejoAsunción Romero
Nov 14, 2016·The Journal of Pain : Official Journal of the American Pain Society·Zhen LiFang Luo
Apr 15, 2011·Korean journal of anesthesiology·Hyong Rae JoYoung Soon Choi
Jan 25, 2018·The European Journal of Neuroscience·Aizhu LuShiyuan Xu
Oct 22, 2020·International Journal of Molecular Sciences·Rosalba SiracusaDaniela Impellizzeri
Apr 29, 2021·European Journal of Pain : EJP·David Cabañero, Rafael Maldonado

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