Increased Src kinase level results in increased protein tyrosine phosphorylation in scrapie-infected neuronal cell lines

FEBS Letters
Hanna GyllbergKatarina Bedecs

Abstract

We have studied how prion infection may affect the Src kinase activity in three different neuronal cell lines, ScGT1 and ScN2a, where ScGT1 were generated in our laboratory. By immunoblotting, using clone 28 - a monoclonal antibody recognizing active Src, we have found a 32+/-6.3% and 75+/-7.7% elevation in Src activity in ScGT1 and ScN2a cells, respectively, compared to uninfected cells. Immunocomplex in vitro kinase assay confirmed the increased Src activity. The increased Src kinase activity in scrapie-infected cells was further shown to correlate to an increased level of Src protein. In addition, an important increase in the protein tyrosine phosphorylation signal was observed in ScGT1 and ScN2a cells, which was further shown to be Src-dependent, as treatment with PP2 - a Src family kinase specific inhibitor, reversed the protein tyrosine phosphorylation profile. Abnormal Src-kinase activation and subsequent protein tyrosine phosphorylation may be key elements in the neuropathology of the prion diseases.

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Citations

Oct 6, 2010·Cell Communication and Signaling : CCS·Wibke WagnerSilja Wessler
May 19, 2010·Molecular Neurodegeneration·Chunjiang YuMary Jo Ladu
Aug 23, 2008·The European Journal of Neuroscience·Kajsa LöfgrenKatrin Mani
Mar 13, 2013·Cellular & Molecular Biology Letters·Alessandro Didonna
Nov 5, 2014·Frontiers in Cell and Developmental Biology·Katharina Ochs, Edward Málaga-Trillo
Feb 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kajsa LöfgrenKatarina Bedecs

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