Increased survival in sepsis by in vivo adenovirus-induced expression of IL-10 in dendritic cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Andreas OberholzerMichael J Clare-Salzler

Abstract

The dendritic cell (DC) is the most potent APC of the immune system, capable of stimulating naive T cells to proliferate and differentiate into effector T cells. Recombinant adenovirus (Adv) readily transduces DCs in vitro allowing directed delivery of transgenes that modify DC function and immune responses. In this study we demonstrate that footpad injection of a recombinant Adv readily targets transduction of myeloid and lymphoid DCs in the draining popliteal lymph node, but not in other lymphoid organs. Popliteal DCs transduced with an empty recombinant Adv undergo maturation, as determined by high MHC class II and CD86 expression. However, transduction with vectors expressing human IL-10 limit DC maturation and associated T cell activation in the draining lymph node. The extent of IL-10 expression is dose dependent; transduction with low particle numbers (10(5)) yields only local expression, while transduction with higher particle numbers (10(7) and 10(10)) leads additionally to IL-10 appearance in the circulation. Furthermore, local DC expression of human IL-10 following in vivo transduction with low particle numbers (10(5)) significantly improves survival following cecal ligation and puncture, suggesting that compartmenta...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·R M Steinman
Oct 1, 1989·Critical Care Medicine·P DamasP Franchimont
Jun 1, 1995·Molecular Biotechnology·F L Graham, L Prevec
Nov 1, 1996·The Journal of Infectious Diseases·T van der PollS F Lowry
May 1, 1997·European Journal of Immunology·T De SmedtM Moser
Apr 1, 1998·Nature·J Banchereau, R M Steinman
May 20, 1998·Critical Care Medicine·D G RemickG L Bolgos
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M MinterL L Moldawer
Mar 13, 2001·Annual Review of Immunology·K W MooreA O'Garra
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S CorintiG Girolomoni
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C OberholzerL L Moldawer
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R S HotchkissI E Karl
May 24, 2001·Immunity·C Reis e Sousa
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M WysockaC L Karp
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R M MinterL L Moldawer
Jul 14, 2001·Science·B PulendranJ Banchereau
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·C OberholzerL L Moldawer

❮ Previous
Next ❯

Citations

May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sabine HovesMartin Fleck
Oct 16, 2003·Shock·Philip Efron, Lyle L Moldawer
May 16, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alex G CuencaLyle L Moldawer
Jul 22, 2005·Journal of Leukocyte Biology·Hironori TsujimotoLyle L Moldawer
Jan 21, 2006·American Journal of Respiratory Cell and Molecular Biology·Ali RoghanianJean-Michel Sallenave
Dec 13, 2005·Critical Care Medicine·Philip O Scumpia, Lyle L Moldawer
Jun 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew J DelanoPhilip A Efron
Feb 11, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A A DurgaryanA A Galoyan
Mar 31, 2015·BioMed Research International·Xia FanHua-ping Liang
Aug 26, 2006·Critical Care Medicine·Jon A BurasWende R Reenstra
Oct 31, 2008·Scandinavian Journal of Immunology·S K TschoekeA Oberholzer
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip O ScumpiaPhilip A Efron
May 30, 2003·Pharmacological Reviews·K AsadullahH D Volk
Apr 26, 2007·Journal of Intensive Care Medicine·Eddy Jean-Baptiste
May 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emmanuel L GautierPhilippe Lesnik
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip A EfronLyle L Moldawer
Mar 17, 2006·Critical Care Medicine·Sven K TschoekeLyle L Moldawer
Oct 15, 2005·Nature Reviews. Drug Discovery·Jon A BurasMichail Sitkovsky
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip O ScumpiaLyle L Moldawer
Oct 28, 2017·Journal of Immunology Research·Dong-Dong WuXin-Ying Ji
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin W TinsleyRichard S Hotchkiss
Mar 3, 2007·Der Orthopäde·S K Tschöke, A Oberholzer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis