PMID: 16523658Mar 10, 2006Paper

Increased survival of preimplantation mouse embryos in medium with recombinant cytokine LIF

Ontogenez
L M MezhevikinaE E Fesenko

Abstract

We studied the effects of cytokine LIF on in vitro development of 2-cell mouse embryos to the late blastocyst stage. LIF at 10 ng/ml enhanced the blastocyst formation and hatching from zona pellucida. When blastocysts were cultivated in a medium with LIF for a longer time, the trophoblast adhesive properties and proliferative activity were enhanced. In the presence of this cytokine, the trophoblast cells were attached to the substrate surface and fulfill the function of a sublayer for growth of the inner cell mass colonies with a high activity of endogenous alkaline phosphatase. Expression of LIF was detected in the oviduct and uterus epithelial tissues from day 1 until day 4 of pregnancy, thus suggesting its involvement in early development. According to the data of cultivation, cytokine LIF enhanced the adhesive properties and functional activity of the trophoblast cells, which is essential for implantation of blastocysts in the uterus.

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Citations

Jan 19, 2008·Journal of Assisted Reproduction and Genetics·Behnaz SheikholslamiMehdi Ramezanzadeh

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