Increased susceptibility to otitis media in a Splunc1-deficient mouse model

Disease Models & Mechanisms
Jennifer A BartlettPaul B McCray

Abstract

Otitis media (inflammation of the middle ear) is one of the most common diseases of early childhood. Susceptibility to otitis is influenced by a number of factors, including the actions of innate immune molecules secreted by the epithelia lining the nasopharynx, middle ear and Eustachian tube. The SPLUNC1 (short palate, lung, nasal epithelial clone 1) protein is a highly abundant secretory product of the mammalian nasal, oral and respiratory mucosa that is thought to play a multifunctional role in host defense. In this study we investigated Splunc1 expression in the ear of the mouse, and examined whether this protein contributes to overall host defense in the middle ear and/or Eustachian tube. We found that Splunc1 is highly expressed in both the surface epithelium and in submucosal glands in these regions in wild-type mice. In mice lacking Splunc1, we noted histologically an increased frequency of otitis media, characterized by the accumulation of leukocytes (neutrophils with scattered macrophages), proteinaceous fluid and mucus in the middle ear lumens. Furthermore, many of these mice had extensive remodeling of the middle ear wall, suggesting a chronic course of disease. From these observations, we conclude that loss of Splu...Continue Reading

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Citations

May 5, 2016·Biochemistry·William G WaltonMatthew R Redinbo
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Nov 11, 2017·Disease Models & Mechanisms·Mahmood F BhuttaMichael T Cheeseman
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Apr 11, 2019·PLoS Pathogens·Kalyan K DewanEric T Harvill
Sep 30, 2020·PloS One·Daniel BodmerSoledad Levano Huaman
Mar 3, 2020·Frontiers in Genetics·Ruishuang GengQing Yin Zheng
Mar 23, 2019·Disease Models & Mechanisms·Jorge Del-PozoMichael Cheeseman
Feb 3, 2020·International Journal of Pediatric Otorhinolaryngology·Regie Lyn P Santos-CortezGarth D Ehrlich
Oct 17, 2015·Disease Models & Mechanisms·Hilda TateossianSteve D M Brown

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Datasets Mentioned

BETA
MAB1897

Methods Mentioned

BETA
bronchoalveolar lavage
fluorescence microscopy

Software Mentioned

GraphPad Prism
Metaview
ImageJ

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