Increased T-cell survival by structural bronchial cells derived from asthmatic subjects cultured in an engineered human mucosa

The Journal of Allergy and Clinical Immunology
Marie-Eve DarveauJamila Chakir

Abstract

Interaction between lymphocytes and structural cells has been proposed as a key factor in regulating inflammation in asthma. This study was designed to investigate the effect of epithelial cells and fibroblasts on T-lymphocyte survival by using a 3-dimensional tissue-engineered model. Engineered human bronchial mucosal tissues were produced by using fibroblasts, epithelial cells, and autologous T cells from asthmatic and healthy donors. T-cell apoptosis and apoptotic marker expression by T cells were evaluated by using the terminal deoxynucleotidyl transferase biotinylated d-UTP nick end-labeling technique and immunofluorescence, respectively. Cytokines implicated in T-cell survival were measured by means of ELISA in culture supernatants. We demonstrated histologically that we were able to generate a well-structured engineered bronchial mucosa by using epithelial cells, fibroblasts, and T cells cultured from healthy and asthmatic subjects. Structural cells from asthmatic subjects cultured in this model induced a significant decrease in the ability of T cells to undergo apoptosis represented by a decrease in DNA fragmentation and proapoptotic molecule expression (Bcl-2-associated X protein and Fas ligand). Structural cells from ...Continue Reading

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Citations

Mar 23, 2010·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Tamas F Molnar, Judit E Pongracz
Oct 22, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Takechiyo YamadaShigeharu Fujieda
Jun 30, 2009·The Journal of Allergy and Clinical Immunology·Emily J SwindleDonna E Davies
Oct 21, 2015·Genomics Data·Dennis LazarevJames L Manley
Jan 15, 2015·Tissue Engineering. Part B, Reviews·Cian O'LearySally-Ann Cryan
Oct 4, 2014·Journal of Cell Death·Pinja IlmarinenHannu Kankaanranta
Jun 24, 2020·Nature Immunology·Ursula SmoleMarsha Wills-Karp

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