Increased thermal stability of the enzyme content in permeabilized whole cells from the fission yeast Schizosaccharomyces pombe by exogenous trehalose and other compounds

Canadian Journal of Microbiology
J FernándezMariano Gacto

Abstract

Cells of the fission yeast Schizosaccharomyces pombe were permeabilized by treatment with toluene-ethanol. The permeabilized cells lost the bulk of the internal trehalose pool while most of the alkaline phosphatase, invertase, alpha-glucosidase, or neutral trehalase activities located inside the cells remained unaffected. This system was used as an in situ assay to determine the involvement of trehalose in enzyme protection during thermal treatments. The addition of trehalose to suspensions of permeabilized cells resulted in a sugar-dependent thermoprotection of the internal marker enzymes. This approach demonstrates that in whole cells of the fission yeast trehalose plays a physiological role as a protective molecule against thermal denaturation of cellular enzymes.

References

Feb 15, 1987·The Biochemical Journal·J H CroweC Aurell Wistrom
Jan 1, 1988·Antonie van Leeuwenhoek·J C Arguelles, M Gacto
Jan 1, 1987·Annual Review of Biophysics and Biophysical Chemistry·W Saenger
Mar 1, 1982·Analytical Biochemistry·H Felix
Aug 1, 1983·Journal of General Microbiology·S P RamM G Shepherd

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Citations

Jul 3, 2020·Genes·Anne M MacKenzie, Soni Lacefield

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