PMID: 6965909Mar 1, 1980Paper

Increased toxicity of diphtheria toxin for human lymphoblastoid cells following covalent linkage to anti-(human lymphocyte) globulin or its F(ab')2 fragment

European Journal of Biochemistry
W C RossA J Davies

Abstract

Anti-(human lymphocyte) globulin was reacted with a mixed anhydride derivative of chlorambucil to give a product which was in turn reacted with diphtheria toxin. The resulting conjugate was partially purified and was found to possess an ability similar to that of the native antibody to bind to the human lymphoblastoid cell lines, CLA4 and Daudi. Daudi cells, as had been observed previously with CLA4 cells, lacked the high sensitivity to diphtheria toxin normally characteristic of cells of human origin. Thus treatment with free toxin at a concentration of 1 microgram/ml was without effect upon their ability to incorporate [3H]leucine. By contrast, Daudi cells were highly sensitive to toxin conjugated to anti-(human lymphocyte) globulin or to its F(ab')2 fragment. Exposure for 24 h to a solution of conjugate containing toxin at a concentration of 0.5 ng/ml caused a reduction of 50% in the leucine uptake by Daudi cells. The toxicity of the conjugate could be blocked by diphtheria antitoxin or by pretreatment of the cells with non-conjugated antibody. Toxin linked to normal horse IgG or to its F(ab')2 fragment was without cytotoxic effect upon Daudi cells. Furthermore both the conjugate with anti-(human lymphocyte) globulin and tha...Continue Reading

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Citations

Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T F BumolN O Kaplan
Jun 1, 1982·The Journal of Experimental Medicine·K A KrolickE S Vitetta
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K A KrolickE S Vitetta
Jan 1, 1983·Pharmacology & Therapeutics·D C Edwards
Jan 1, 1981·Pharmacology & Therapeutics·S Olsnes, A Pihl
Feb 1, 1996·Cancer Biotherapy & Radiopharmaceuticals·E M Rakowicz-SzulczynskaM L Smith
Jan 1, 1982·Immunological Reviews·P E Thorpe, W C Ross
Jan 1, 1982·Immunological Reviews·E S VitettaJ W Uhr
Jan 1, 1982·Immunological Reviews·F L MooltenS H Zajdel
Mar 12, 1981·Nature·S Olsnes
Aug 26, 2020·Blood Advances·Naveen Pemmaraju, Marina Konopleva
Jan 1, 1986·Journal of Immunopharmacology·N E KaminskiF E Guthrie
Oct 15, 1982·International Journal of Cancer. Journal International Du Cancer·P CasellasK E Hellström
May 3, 2018·Pathogens and Disease·Jean-Marc Cavaillon
Apr 29, 1983·Journal of Immunological Methods·A H Blair, T I Ghose
Jun 3, 2021·International Journal of Molecular Sciences·Olga ShilovaSergey Deyev
Aug 1, 1981·Lancet·G Gregoriadis
Feb 11, 1983·Science·E S VitettaJ W Uhr

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