PMID: 9662275Jul 14, 1998Paper

Increased tyrosine phosphorylation of band 3 in hemoglobinopathies

American Journal of Hematology
H T TerraS T Saad

Abstract

In order to investigate the tyrosine phosphorylation of band 3, we performed immunoblotting of intact red cells using anti-phosphotyrosine antibody of 21 patients with sickle cell disorders (11 SS, 5 Sbeta, 5 SC), 7 patients with beta thalassemias (5 beta thal intermedia, 2 deltabeta thal), 10 normal controls, and 1 patient with hereditary spherocytosis. They had not received transfusion for the last 4 months and all were clinically stable. Our results showed an increased tyrosine phosphorylation of two proteins, in the 100 and 80 kD regions, in sickle cell and beta-thalassemic red cells when compared to the normal controls and to the patient with hereditary spherocytosis. Immunoprecipitation of the lysed red cells with anti-band 3 antibody and immunoblotting with anti-phosphotyrosine antibody confirmed that the 100 kD tyrosine phosphorylated protein was band 3. In the sickle cell disease group, the band 3 tyrosine phosphorylation varied from 2- to 10-fold increase compared to control (x +/- SD; SS = 7.8- +/- 2.7-fold; SC = 3.8- +/- 1.3-fold; Sbeta = 5.2- +/- 2.0-fold). It was also higher in the beta-thalassemic group (beta-thal = 4.3- +/- 3.7-fold). There was no significant difference in tyrosine phosphorylation among the vari...Continue Reading

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Citations

Nov 2, 2013·Future Medicinal Chemistry·Françoise Nepveu, Francesco Turrini
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Diana TetiLeonardo Romano
Mar 27, 2007·Biochimica Et Biophysica Acta·Michael R CondonZoltan Spolarics
Jan 21, 2018·American Journal of Hematology·Slim AzouziCaroline Le Van Kim
Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Estela Puchulu-CampanellaPhilip S Low
Apr 30, 2020·British Journal of Haematology·Panae NoomunaPhilip S Low
Jun 19, 2018·Frontiers in Physiology·Rick HuisjesRichard van Wijk

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