Increased ubiquitination and other covariant phenotypes attributed to a strain- and temperature-dependent defect of reovirus core protein mu2

Journal of Virology
Cathy L MillerMax L Nibert

Abstract

Reovirus replication and assembly are thought to occur within cytoplasmic inclusion bodies, which we call viral factories. A strain-dependent difference in the morphology of these structures reflects more effective microtubule association by the mu2 core proteins of some viral strains, which form filamentous factories, than by those of others, which form globular factories. For this report, we identified and characterized another strain-dependent attribute of the factories, namely, the extent to which they colocalized with conjugated ubiquitin (cUb). Among 16 laboratory strains and field isolates, the extent of factory costaining for cUb paralleled factory morphology, with globular strains exhibiting higher levels by far. In reassortant viruses, factory costaining for cUb mapped primarily to the mu2-encoding M1 genome segment, although contributions by the lambda3- and lambda2-encoding L1 and L2 genome segments were also evident. Immunoprecipitations revealed that cells infected with globular strains contained higher levels of ubiquitinated mu2 (Ub-mu2). In M1-transfected cells, cUb commonly colocalized with aggregates formed by mu2 from globular strains but not with microtubules coated by mu2 from filamentous strains, and immu...Continue Reading

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Citations

Jan 30, 2009·Journal of Virology·Takeshi KobayashiTerence S Dermody
Apr 24, 2015·Frontiers in Cellular and Infection Microbiology·Peyman EzzatiKevin M Coombs
Mar 7, 2007·Molecular & Cellular Proteomics : MCP·Cathy L MillerMax L Nibert
Jan 13, 2017·Journal of Virology·Efraín E Rivera-SerranoBarbara Sherry

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