Increased urinary excretion of orosomucoid is a risk predictor of diabetic nephropathy

Nephrology
Hongjuan JiangJing Cheng

Abstract

Identification of the risk predictor of diabetic nephropathy (DN) remains a major challenge currently. Thus, proteomic approaches to identify DN-related biomarker were performed. A comparative proteomic approach of 2-D gel electrophoresis (2-DE) and mass spectrometry to identify biomarkers in urine samples from 12 DN patients (six type 1 and six type 2 diabetic patients) and six healthy controls. Then, the urinary level of identified protein biomarker was detected by immunoturbidimetry assay in urine samples from 90 type 1 and type 2 diabetic patients with normo-, micro- and macroalbuminuria (n = 30 in each group), and 30 healthy controls. A novel DN-related protein, orosomucoid (alpha1-acid glycoprotein), was identified by proteomic method. Its abundance was highly upregulated (>eightfold) in DN patients. The data of immunoturbidimetry assay showed urinary orosomucoid excretion rate (UOER) was gradually increased in the normo-, micro- and macroalbuminuria group versus control (0.71 +/- 0.41, 1.93 +/- 0.68, 2.88 +/- 0.94 vs 0.39 +/- 0.28 ug/min, P < 0.05). The result indicated that UOER increased in early stage of DN and gradually increased with the development of DN. Also, Pearson's correlation analysis indicated UOER was posi...Continue Reading

References

Mar 1, 1979·European Journal of Applied Physiology and Occupational Physiology·J R Poortmans, G Haralambie
Nov 4, 2000·Biochimica Et Biophysica Acta·T FournierD Porquet
Nov 4, 2000·Biochimica Et Biophysica Acta·L Lögdberg, L Wester
Aug 28, 2001·Diabetes Care·B P TabaeiW H Herman
Jun 28, 2003·Diabetes·Bruce B DuncanUNKNOWN Atherosclerosis Risk in Communities Study
Dec 3, 2003·Contributions to Nephrology·Visith ThongboonkerdKenneth R McLeish
Jul 30, 2004·Clinical Science·Harald MischakDanilo Fliser
Feb 10, 2007·Expert Review of Proteomics·Hassan Dihazi, Gerhard A Müller
Feb 16, 2007·Journal of the American Society of Nephrology : JASN·Sanju A VargheseJohn M Arthur
Mar 1, 2007·Diabetes Care·Paturi V RaoSrinivasa R Nagalla
Jul 13, 2007·Journal of the American Society of Nephrology : JASN·Michael S GoligorskyEdmond O'Riordan
Feb 8, 2008·Journal of the American Society of Nephrology : JASN·Juan F Navarro-González, Carmen Mora-Fernández

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Citations

May 18, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Randa Ben AmeurClaude Granier
Oct 3, 2009·American Journal of Respiratory and Critical Care Medicine·David GozalOscar Sans Capdevila
Mar 5, 2014·Disease Markers·Shuk-Man KaAnn Chen
Sep 22, 2012·Proteomics. Clinical Applications·Ana Isabel PadrãoFrancisco Amado
Mar 30, 2010·Journal of Zhejiang University. Science. B·Jing WuWei Gu
Jul 15, 2016·Journal of Diabetes Research·Cristina GluhovschiBogdan Timar
Mar 16, 2016·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Nagham M S El-BeblawyMarwa A Erfan
Nov 20, 2018·European Journal of Clinical Investigation·Balázs SzirmayTamás Kőszegi
Jan 8, 2014·Experimental and Therapeutic Medicine·Xiao-Qin TianYan-Cheng Xu
Sep 29, 2017·Critical Reviews in Clinical Laboratory Sciences·Nektaria Papadopoulou-MarketouIoannis Papassotiriou
Nov 7, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Péter KustánDiána Mühl
Jun 24, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Naseer Ullah KhanLiming Shen

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