Increased VGLUT3 involved in visceral hyperalgesia in a rat model of irritable bowel syndrome

World Journal of Gastroenterology : WJG
Chang-qing YangLi-Li Guo

Abstract

To investigate the activity of vesicular glutamate transporter-3 (VGLUT3) in a visceral hyperalgesia rat model of irritable bowel syndrome, and the role of mast cells (MCs). Transient intestinal infection was induced by oral administration of Trichinella spiralis larvae in rats. On the 100(th) day post-infection (PI), the rats were divided into an acute cold restraint stress (ACRS) group and a non-stressed group. Age-matched untreated rats served as controls. The abdominal withdrawal reflex was used to measure the visceromotor response to colorectal distension (CRD). The expression levels of VGLUT3 in peripheral and central neurons were analyzed by immunofluorescence and western blotting. VGLUT3 expression in the L6S1 dorsal root ganglion cells was significantly higher in the PI group than in the control group (0.32 ± 0.009 vs 0.22 ± 0.008, P < 0.01), and there was no significant difference in the expression of VGLUT3 between MC-deficient rats and their normal wild-type littermates. Immunofluorescence showed that the expression levels of VGLUT3 in PI + ACRS rats were enhanced in the prefrontal cortex of the brain compared with the control group. VGLUT3 is involved in the pathogenesis of visceral hyperalgesia. Coexpression of c-...Continue Reading

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Citations

Dec 8, 2017·Frontiers in Systems Neuroscience·Beverley Greenwood-Van Meerveld, Anthony C Johnson
Jan 10, 2018·Stress : the International Journal on the Biology of Stress·Diána BalázsfiDóra Zelena

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