PMID: 9436467Jun 1, 1997Paper

Increases in cytosolic Ca2+ accompany basophil activation by eosinophil granule major basic protein

Immunology Letters
D W MacGlashanLarry L Thomas

Abstract

The response of basophil cytosolic free Ca2+ concentrations ([Ca2+]i) to stimulation by eosinophil granule major basic protein (MBP) was assessed using digital video microscopy. MBP stimulated an elevation in basophil [Ca2+]i that, in general, developed slowly and increased monotonically. In some experiments, however, the average rise in [Ca2+]i was biphasic, with an initial transient increase followed by a larger and more sustained elevation. At the single cell level MBP stimulation caused frequent, asynchronous oscillations in [Ca2+]i that ranged up to 300 nM in amplitude. Chelation of extracellular Ca2+ selectively blocked the second, sustained rise in [Ca2+]i as well as MBP-induced histamine release. Within individual experiments, the increase in [Ca2+]i paralleled the level of histamine release. These results demonstrate that, unlike the activation of neutrophils or eosinophils, basophil activation by MBP correlates with an elevation in [Ca2+]i. Further, the MBP induced [Ca2+]i response exhibits several features in common with the IgE-mediated [Ca2+]i response.

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Citations

Jan 10, 2004·The Journal of Allergy and Clinical Immunology·Kristin M Leiferman, Gerald J Gleich
Feb 16, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G Beyer-SehlmeyerJ Bertram
Jul 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Boris MussetThomas E DeCoursey
Dec 1, 1994·The Journal of Allergy and Clinical Immunology·L L ThomasY Bilimoria

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