Increasing content of poly A(+) mRNA of serum-stimulated cells in the absence of ribosome synthesis

Journal of Cellular Physiology
L F JohnsonH Green

Abstract

When 3T6 cells undergo a serum-induced transition from resting to growing state, the number of ribosomes and the amounts of mRNA increase as the cells prepare for DNA synthesis. We have examined the effect of preventing ribosome synthesis during this transition. When resting cells are stimulated to grow in the presence of 5-fluorouridine, mRNA accumulates normally during the first eight hours, though new ribosome formation is completely blocked by the drug. At later times, mRNA continues to accumulate, but at a reduced rate. The ratio of poly A(+) mRNA to rRNA increases from the value characteristic of resting 3T6 (1.8%) to that of growing 3T6 (2.7%) by five hours, and continues to increase to abnormally high values after this time. Although labelling of tRNA is not affected after brief exposure of cells to fluorouridine, the drug prevents the later accumulation of tRNA that ordinarily occurs following serum stimulation of resting cells. This failure of accumulation is not the result of increased lability of fluorinated tRNA, but is probably due to failure of the transcription rate of pre-tRNA to increase. It is possible that this effect might be due to a regulatory system coupling tRNA content to ribosome content. In cultures ...Continue Reading

References

Nov 14, 1973·Biochimica Et Biophysica Acta·J A Hassell, D L Engelhardt
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P S Rudland
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J C Mauck, H Green
Oct 1, 1971·Journal of Cellular Physiology·H M Temin

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Citations

Jan 1, 1982·Advances in Enzyme Regulation·J M BuchananR J Smith
Nov 1, 1979·Journal of Cellular Physiology·M L Smith, J M Buchanan
Jul 1, 1980·Journal of Cellular Physiology·D E ChadwickI Lieberman
Jul 1, 1977·Journal of Cellular Physiology·L F Johnson, R Meister

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