Increasing genotypic and phenotypic selection from the original genomic RNA populations of HIV-1 strains LAI and MN (NM) by peripheral blood mononuclear cell culture, B-cell-line propagation and T-cell-line adaptation

AIDS
V V Lukashov, J Goudsmit

Abstract

To address the question of whether T-cell-line adaptation of the original LAI and MN (NM) HIV-1 populations biased the interpretation of the intraindividual and population-wide virus distributions. HIV-1 genomic RNA coding for the gp120 C2V3 region was obtained from serum samples of patients LAI and MN and compared to the proviral DNA derived from simultaneously sampled peripheral blood mononuclear cells (PBMC) as well as B-and T-cell lines. Two (10%) of 20 clones of HIV-1 LAI RNA and none of 16 clones of the HIV-1 MN RNA carried syncytium-inducing (SI)-determining amino-acid changes. HIV-1 LAI RNA formed on SI and two non-SI (NSI) phylogenetic clusters. The HIV-1 LAI DNA in PBMC included both SI and NSI clones but lacked one NSI cluster and contributed NSI clones to the SI cluster as well as an SI clone to the NSI cluster, indicating the existence of an intermediate SI/NSI genotype. In vitro culture using either primary cells or B-cell lines yielded only SI clones, distributed over two SI/NSI mixed clusters. Long-term propagation in T-cell lines further restricted the clonality and yielded SI clones belonging to only one cluster. On the population level, HIV-1 LAI, MN and BRU sequences all clustered according to the individual...Continue Reading

Citations

Mar 5, 1999·Immunology Letters·J A McKeating, P Balfe
Jan 22, 2000·Journal of Virological Methods·L HoltermanJ Heeney
Sep 18, 2001·AIDS Research and Human Retroviruses·I PandreaC Apetrei
Oct 31, 2003·AIDS Research and Human Retroviruses·Fuat KurbanovMitsunobu Imai
Jul 1, 1996·AIDS Research and Human Retroviruses·V V LukashovJ Goudsmit
Jan 22, 2004·British Journal of Cancer·L R HiornsB Leyland-Jones

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