Increasing glycolytic flux in Torulopsis glabrata by redirecting ATP production from oxidative phosphorylation to substrate-level phosphorylation

Journal of Applied Microbiology
L M LiuJ Chen

Abstract

This study aimed at further increasing the pyruvate productivity of a multi-vitamin auxotrophic yeast Torulopsis glabrata by redirecting ATP production from oxidative phosphorylation to substrate-level phosphorylation. We examined two strategies to decrease the activity of F0F1-ATPase. The strategies were to inhibit F0F1-ATPase activity by addition of oligomycin, or to disrupt F0F1-ATPase by screening neomycin-resistant mutant. The addition of 0.05 mmol l(-1) oligomycin to the culture broth of T. glabrata CCTCC M202019 resulted in a significantly decreased intracellular ATP level (35.7%) and a significantly increased glucose consumption rate (49.7%). A neomycin-resistant mutant N07 was screened and selected after nitrosoguanidine mutagenesis of the parent strain T. glabrata CCTCC M202019. Compared with the parent strain, the F0F1-ATPase activity of the mutant N07 decreased about 65%. As a consequence, intracellular ATP level of the mutant N07 decreased by 24%, which resulted in a decreased growth rate and growth yield. As expected, glucose consumption rate and pyruvate productivity of the mutant N07 increased by 34% and 42.9%, respectively. Consistently, the activities of key glycolytic enzymes of the mutant N07, including phos...Continue Reading

References

Jan 1, 1975·Methods in Enzymology·A AustC H Suelter
Mar 1, 1979·European Journal of Biochemistry·C BursteinA Kepes
Jan 1, 1975·Methods in Enzymology·E A Barnard
Dec 1, 1992·Journal of Bacteriology·P R Jensen, O Michelsen
Jul 1, 1989·Yeast·I SchaaffF K Zimmermann
Jan 1, 1988·Physiological Reviews·A E Senior
Jan 1, 1986·Methods in Enzymology·P E Stanley
Oct 20, 1980·FEBS Letters·T Tsuchiya, B P Rosen
Oct 1, 1980·Antimicrobial Agents and Chemotherapy·J J Lipsky, P S Lietman
Mar 15, 1997·The Biochemical Journal·C StefanelliC M Caldarera
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·K WuP Siekevitz
Mar 17, 1998·Biochemical and Biophysical Research Communications·A StruglicsJ F Allen
Feb 24, 2001·Genome Biology·M C Wildermuth
Feb 24, 2001·Nature·A SchmidB Witholt
Jun 26, 2002·Journal of Bacteriology·Brian J KoebmannPeter R Jensen
Aug 16, 2002·European Journal of Biochemistry·Leighton Pritchard, Douglas B Kell
Jun 1, 1961·Journal of Biochemistry·F HUIJING, E C SLATER

❮ Previous
Next ❯

Citations

Jul 26, 2013·Critical Reviews in Biotechnology·Shubo LiJian Chen
Nov 26, 2008·Biotechnology Advances·Jingwen ZhouJian Chen
Oct 19, 2017·Biotechnology and Bioengineering·Zhengshan LuoJian Chen
Mar 12, 2019·ACS Synthetic Biology·Zhengshan LuoJingwen Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.