PMID: 8947587Sep 1, 1996Paper

Increasing methylation of the calcitonin gene during disease progression in sequential samples from CML patients

Leukemia Research
K I MillsA K Burnett

Abstract

In chronic myeloid leukaemia (CML), disease progression from the initial chronic phase to the acute phase or blast crisis has previously been shown to be correlated with progressive increases in hyper-methylation of the calcitonin gene, located at chromosome 11p15. However, sequential studies of individual patients were not performed in these investigations. We have analysed 44 samples from nine patients with typical Philadelphia chromosome positive CML throughout their disease progression to determine the methylation state of the calcitonin gene at these time points. Densitometry was used to quantitate the ratio of the normal 2.0 kb Hpa II fragments, indicating normal methylation status of the gene, compared to the intensity of the abnormal, hyper-methylated, 2.6-3.1 kb Hpa II fragments. We found a gradual increase in the ratio of methylated:unmethylated calcitonin gene during chronic phase with a dramatic rise at blast crisis. Further, the ratio of the abnormal hypermethylated 3.1 kb fragments to the methylated 2.6 kb fragment resulted in the identification of a clonal expansion of abnormally methylated cells. This expansion of cells with hypermethylation of the calcitonin gene during chronic phase was shown to coincide with ...Continue Reading

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H AhujaM J Cline
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A de BustrosS B Baylin
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R Dalla-FaveraF Wong-Staal

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Citations

Dec 1, 1996·Blood Reviews·B H RamsahoyeK I Mills
Jul 27, 2002·Cancer Letters·Laura J Rush, Christoph Plass
Mar 19, 2013·Genetic Testing and Molecular Biomarkers·Qin ChenQing Liu
Dec 1, 2011·Epigenomics·Cristina FloreanMarc Diederich
Mar 23, 2011·Journal of Pediatric Hematology/oncology·Eman Abdel Rahman IsmailRania Ali Hassan El-Farrash
Aug 6, 2019·Frontiers in Cell and Developmental Biology·Jane BuglerDavid Vetrie

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