Increasing of oxidative stress from mitochondria in type 2 diabetic patients

Diabetes/metabolism Research and Reviews
Shuhei NakanishiNobuoki Kohno

Abstract

Recent evidence increasingly indicates that oxidative stress may play an important role in the pathogenesis of diabetic vascular complications. Mitochondria has received much attention as an important organ in the generation of oxidative stress. However, the importance of oxidative stress among diabetic patients without vascular complications is unclear. We compared oxidative stress produced from mitochondria of the mononuclear cells in peripheral blood obtained from 26 diabetic subjects without clinical vascular complications and 52 healthy age-matched subjects using a flow cytometer. Oxidative stress from the mononuclear cells was evaluated by measuring fluorescence of oxidized production from dihydrorhodamine-123, which is a pro-fluorescent compound that selectively accumulates in the mitochondria of living cells. Stimulation of the cells was carried out with phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator. We then calculated the relative fluorescence variation (RFV) that indicated an increasing rate of oxidative stress levels by stimulation with PMA against the levels obtained at baseline. Additionally, we measured the urinary stress markers, 8-hydroxydeoxyguanosine (8OHdG) and 8-epi-prostaglandin ...Continue Reading

Citations

Mar 25, 2009·Journal of Clinical Biochemistry and Nutrition·Noriko SakanoKeiki Ogino
Aug 26, 2014·Oxidative Medicine and Cellular Longevity·Kamila SyslováPetr Kačer
May 24, 2011·Clinical Biochemistry·Chunfang QiuMichelle A Williams
Jan 15, 2008·Metabolism: Clinical and Experimental·Hideki NojimaUNKNOWN Hiroshima University Health Promotion Study Group
Apr 21, 2006·Diabetes Research and Clinical Practice·Tomokazu OyamaKohji Shirai
Dec 4, 2010·Free Radical Biology & Medicine·Sean S Davies, L Jackson Roberts
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Oct 18, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kohei NonakaJun-Ichi Kido
May 30, 2020·International Journal of Molecular Sciences·Melanie GrailleNancy B Hopf
Apr 22, 2018·Molecular and Cellular Biochemistry·Julia Matzenbacher Dos SantosSandra Aparecida Benite-Ribeiro

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