Increasing ornithine decarboxylase activity is another way of prolactin preventing methotrexate-induced apoptosis: crosstalk between ODC and BCL-2

Apoptosis : an International Journal on Programmed Cell Death
P-C HsuG-Y Liu

Abstract

Prolactin has more than 300 separate functions including affecting mammary growth, differentiation, secretion and anti-apoptosis. In the previous studies, prolactin induced Bcl-2 expression to prevent apoptosis and also provoked the activity of ornithine decarboxylase (ODC). Our previous data showed that ODC overexpression upregulates Bcl-2 and prevents tumor necrosis factor alpha (TNF-alpha)- and methotrexate (MTX)-induced apoptosis. Here, we further investigate whether prolactin prevents MTX-induced apoptosis through inducing ODC activity and the relationship between ODC and Bcl-2 upon prolactin stimulation. Prolactin prevented MTX-induced apoptosis in a dose-dependent manner in HL-60 cells. Following prolactin stimulation, ODC enzyme activity also shows an increase in a dose-dependent manner, expressing its maximum level at 3 h, and rapidly declining thereafter. Prolactin-induced ODC activity is completely blocked by a protein kinase C delta (PKCdelta) inhibitor, rottlerin. However, there are no changes in the expressions of ODC mRNA and protein level after prolactin stimulus. It indicates that prolactin may induce ODC activity through the PCKdelta pathway. Besides, Bcl-2 expresses within 1 h of prolactin treatment and this ...Continue Reading

References

Apr 10, 1992·European Journal of Pharmacology·M D SauroN E Zorn
Jan 2, 1990·Molecular and Cellular Endocrinology·L Y Yu-Lee
May 1, 1989·Molecular and Cellular Endocrinology·S B Waters, J A Rillema
Feb 1, 1988·Archives of Biochemistry and Biophysics·J L MitchellM F Hicks
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T G O'BrienM Holmes
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Kahana, D Nathans
Jan 28, 1971·Annals of the New York Academy of Sciences·R A PattilloR F Mattingly
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Aug 16, 1984·Biochemical and Biophysical Research Communications·F MeggioL A Pinna
Nov 1, 1982·The American Journal of Physiology·A E Pegg, P P McCann
Jul 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·H A LaVoie, R J Witorsch
Dec 1, 1996·Endocrine Reviews·N Ben-JonathanR W Steinmetz
Jul 17, 1998·The Journal of Clinical Investigation·L GenestierJ P Revillard
Sep 2, 1998·Transplantation Proceedings·R PaillotJ P Revillard
Feb 27, 1999·Biochemical Society Transactions·L PerssonJ Holmgren
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·J L WangZ Huang
Nov 21, 2000·Rheumatic Diseases Clinics of North America·S E Walker, J D Jacobson
Dec 9, 2000·Analytical Biochemistry·Y Wang, U Bachrach
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·P Coffino
Apr 6, 2001·Cellular and Molecular Life Sciences : CMLS·T Thomas, T J Thomas
Feb 26, 2003·European Urology·Alain RuffionPauline R M Dobson
Apr 17, 2003·Endocrinology·Nithya KrishnanArthur R Buckley
Aug 2, 2003·Archives of Biochemistry and Biophysics·M Laura FernándezCarlota Wolfenstein-Todel
Dec 4, 2003·Apoptosis : an International Journal on Programmed Cell Death·T PloszajL Zwierzchowski
Jun 24, 2004·British Journal of Cancer·C M PerksJ M P Holly
May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·G-Y LiuH-C Hung
Sep 1, 2005·Apoptosis : an International Journal on Programmed Cell Death·C -C HuangG Y Liu

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Jul 29, 2009·The Journal of Biological Chemistry·Kuo-Liang SuGuang-Yaw Liu

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